Comparative analysis of antioxidant activities and phenolic contents in selected wild edible plants

Main Article Content

Ertan Yıldırım https://orcid.org/0000-0003-3369-0645
Melek Ekinci https://orcid.org/0000-0002-7604-3803
Metin Turan https://orcid.org/0000-0002-4849-7680
Elif Maskan https://orcid.org/0009-0002-4920-2343
Halil İbrahim Binici

Keywords

Wild edible plants, antioxidant capacity, phenolic compounds, functional foods

Abstract

This study presents a comparative evaluation of the antioxidant activities and phenolic contents of widely consumed wild edible plants in Türkiye. Various biochemical parameters, including moisture content, ash content, pH, titratable acidity, total phenolic content (TPC), total flavonol (Tflavonol), and total flavonoid (Tflavonoid) content, were determined alongside antioxidant activities assessed by FRAP, DPPH, ABTS, and CUPRAC assays. Significant variations were observed among the plant species. Polygonum alpinum exhibited the highest TPC (66.27 mg GAE/100 g) and CUPRAC antioxidant capacity (38.08 mg TEAC/100 g), whereas Hylotelephium telephium showed the lowest antioxidant activity (TPC: 7.99 mg GAE/100 g; ABTS: 7.11 mg TEAC/100 g). Urtica dioica demonstrated notable antioxidant activity, particularly in the FRAP (10.74 mg TEAC/100 g) and DPPH (7.89 mg TEAC/100 g) assays. Principal component analysis (PCA) effectively distinguished Heracleum spondylium, Rumex tuberosus, and Polygonum alpinum from the other species, clustering them based on their elevated antioxidant and phenolic content. The findings emphasize the nutritional significance and functional food potential of these wild plants, offering valuable insights for future applications in the food and health industries.

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References

Alan O., Turk B., Sen F. 2022. A comparative study on the quality attributes, phenolic content and antioxidant activity of cultivated and wild asparagus as influenced by seasonal variations. J. Agric. Sci. 160(6): 483–492. https://doi.org/10.1017/S0021859622000569
Almaraz-Abarca N., da Graça Campos M., Avila-Reyes J. A., Naranjo-Jimenez N., Corral J. H., Gonzalez-Valdez L.S. 2007. Antioxidant activity of polyphenolic extract of monofloral honeybee-collected pollen from mesquite (Prosopis juliflora, Leguminosae). J. Food Compos. Anal. 20(2): 119–124. https://doi.org/10.1016/j.jfca.2006.08.001
Apak R., Güçlü K., Özyürek M., Karademir S.E. 2004. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem. 52(26): 7970–7981. https://doi.org/10.1021/jf048741x
Avci E., Avcı G.A., Kose D.A., Emniyet A.A., Suicmez M. 2014. In vitro antimicrobial and antioxidant activities and GC/MS analysis of the essential oils of Rumex crispus and Rumex cristatus. Hacettepe J. Biol. Chem. 42(2): 193–199. https://doi.org/10.15671/HJBC.201444210855
Binici, H.İ., Şat, İ.G., Yilmaz B. 2024. Comparison of antioxidant, phenolic profile, melatonin, and volatile compounds of some selected plant samples. Food Sci. Nutr. 12(10): 7158–7165. https://doi.org/10.1002/fsn3.4334
Cemeroğlu B.S. 2007. Gıda Analizleri. Gıda Teknolojisi Derneği Yayınları. Ankara Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü. No: 34.
Çolakoğlu M. and Bilgir B. 1977. Ege Bölgesi'nde insan beslenmesinde kullanılan bazı yabani (sarmaşık, stifno, helvacık, deniz börülcesi, ısırgan ve gelincik) otları üzerinde araştırmalar. VI. Bilim Kongresi Tarım ve Ormancılık Araştırma Grubu Tebliğleri. 3: 11–19.
Çolakoğlu M. and Tömek S. 1975. Ege Bölgesi'nde bazı yenebilen otların bileşimleri. Ege Üniversitesi, Ziraat Fakültesi Yayınları. 228: 1–24.
Dalla Costa V., Piovan A., Filippini R., Brun P. 2024. From ethnobotany to biotechnology: wound healing and anti-inflammatory properties of Sedum telephium L. in vitro cultures. Molecules. 29(11): 2472. https://doi.org/10.3390/molecules29112472
Ereifej K.I., Feng H., Rababah T., Almajwal A., Alu'datt M., Gammoh S.I., et al. 2015. Chemical composition, phenolics, anthocyanins concentration and antioxidant activity of ten wild edible plants. FNS. 6(7): 581–590. https://doi.org/10.4236/fns.2015.67061
Feduraev P., Skrypnik L., Nebreeva S., Dzhobadze G., Vatagina A., Kalinina E., et al. 2022. Variability of phenolic compound accumulation and antioxidant activity in wild plants of some Rumex species (Polygonaceae). Antioxidants, 11(2): 311. https://doi.org/10.3390/antiox11020311
Garsiya E.R., Konovalov D.A., Shamilov A.A., Glushko M.P., Orynbasarova K.K. 2019. Traditional medicine plant, Onopordum acanthium L.(Asteraceae): chemical composition and pharmacological research. Plants. 8(2): 40. https://doi.org/10.3390/plants8020040
Göçer H. and Gülçin İ. 2011. Caffeic acid phenethyl ester (CAPE): correlation of structure and antioxidant properties. Int. J. Food Sci. Nutr. 62(8): 821–825. https://doi.org/10.3109/09637486.2011.585963
Gülçin İ., Elmastaş M., Aboul-Enein H.Y. 2012. Antioxidant activity of clove oil-A powerful antioxidant source. Arab. J. Chem. 5(4): 489–499. https://doi.org/10.1016/j.arabjc.2010.09.016
Jiang N., Doseff A.I., Grotewold E. 2016. Flavones: from biosynthesis to health benefits. Plants. 5(2): 27. https://doi.org/10.3390/plants5020027
Karakas H., Cagman Z., Kizilarslan-Hancer C., Erol E. 2025. Isolation and characterization of secondary metabolites from endemic and edible polygonum sivasicum with in vitro antioxidant and cytotoxic activities. ACS Omega. 10(9): 9756–9767. https://doi.org/10.1021/acsomega.5c00438
Keleş F. 1983. ‘Meyve ve Sebze İşleme Teknolojisi Laboratuvar Notları’, Atatürk Üniv. Ziraat Fak., Erzurum.
Khan T.M. and Kakde U.B. 2014. Biodiversity in wild vegetables of Konkan region-Maharashtra. Int. J. Res. Biosci. Agric. Technol. 2: 226–240.
Köksal E., Gülçin I., Beyza S., Sarikaya Ö., Bursal E. 2009. In vitro antioxidant activity of silymarin. J. Enzyme Inhib. Med. Chem. 24(2): 395–405. https://doi.org/10.1080/14756360802188081
Kordali Ş., Bozhüyük A.U., Beyzi E., Güneş A., Turan M. 2021. Antioxidant enzyme, phenolic substance and plant nutrient contents of Malva sylvestris L. and Alcea rosea L. species used as medicinal plants.
Mwamatope B., Chikowe I., Tembo D.T., Kamanula J.F., Masumbu F.F., Kumwenda F.D. 2023. Phytochemical composition and antioxidant activity of edible wild fruits from malawi. BioMed Res. Int. 2023(1): 2621434. https://doi.org/10.1155/2023/2621434
Ozsoy N., Yilmaz-Ozden T., Aksoy-Sagirli P., Şahin H. Sarı A. 2018. Antioxidant, anti-acetylcholinesterase, anti-inflammatory and DNA protection activities of glaucium grandiflorum var. grandiflorum. IJPR. 17(2): 677.
Öztürk H.I., Nas H., Ekinci M., Turan M., Ercisli S., Narmanlioglu H.K., et al. 2022. Antioxidant activity, phenolic composition, and hormone content of wild edible vegetables. Horticulturae. 8(5): 427. https://doi.org/10.3390/horticulturae8050427
Pinela J., Carvalho A.M., Ferreira I.C. 2017. Wild edible plants: nutritional and toxicological characteristics, retrieval strategies and importance for today’s society. FCT. 110: 165–188. https://doi.org/10.1016/j.fct.2017.10.020
Rice-Evans C., Miller N., Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci. 2(4): 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
Samancıoğlu A., Sat I.G., Yıldırım E., Ercişli S., Jurikova T., Mıcek T. 2016. Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. Indian J. Tradit. Knowl. 15(2): 208–213.
Samtiya M., Aluko R.E., Dhewa, T., Moreno-Rojas J.M. 2021. Potential health benefits of plant food-derived bioactive components: an overview. Foods. 10(4): 839. https://doi.org/10.3390/foods10040839
Şekeroğlu N. and Gezici S. 2020. Koronavirüs pandemisi ve Türkiye'nin bazı şifalı bitkileri. Anatol. Clin. 25(Special Issue on COVID 19): 163–182. https://doi.org/10.21673/anadoluklin.724210
Spada P.D., de Souza G.G.N., Bortolini G.V., Henriques J.A., Salvador M. 2008. Antioxidant, mutagenic, and antimutagenic activity of frozen fruits. J. Med. Food. 11(1): 144–151.
https://doi.org/10.1089/jmf.2007.598
Stankovic M.S. 2011. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J. Sci. 33(2011): 63–72.
Wrolstad R.E. 1993. Color and pigment analyses in fruit products. Oregon State University, 624.
Youssef K.M. and Mokhtar S.M. 2014. Effect of drying methods on the antioxidant capacity, color and phytochemicals of Portulaca oleracea L. leaves. J. Nutr. Food Sci. 4(6): 1. https://doi.org/10.4172/2155-9600.1000322