Valorisation of date fruits by-products for the production of biopolymer polyhydroxybutyrate (PHB) using the bacterial strain Bacillus paramycoides
Main Article Content
Keywords
Bacillus paramycoides, bioprocess, date syrup, Polyhydroxybutyrate (PHB), valorization
Abstract
The aim of this research was to study the valorization of the date fruit by-product by conversion of date syrup into biopolymer polyhydroxybutyrate (PHB), based on the metabolic capacity of the bacterial strain Bacillus paramycoides to accumulate PHB from date syrup. Total reducing sugars in date syrup was essayed using 3',5-dinitrosalicylic acid (DNS) and HPLC methods. Bacillus paramycoides was isolated from soil of the botanic garden of Skikda university, Algeria. The accumulated PHB was extracted using chloroform. It was quantified as crotonic acid in concentrated sulfuric acid (H2SO4) by spectrophotometry at 300nm. Date syrup is characterized by high levels of total sugars (79.66 g/L) with 31.86 g/L of total reducing sugars. PHB accumulation reached its maximum (104.3 ug/mL) after 96 h of incubation at pH 7 and temperature 37°C using Tryptophane as the nitrogen source and acid pretreated syrup at a concentration of 8%. HPLC analysis on Aminex HPX-87H showed that the produced PHB from date syrup is characterized by a chromatogram peak with a retention time at 22.5 min.
References
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Aly, M.M., Albureikan, M., El Rabey, H. and Kabli, S.A., 2013. Effects of culture conditions on growth and poly–beta-hydroxybutyric acid production by Bacillus cereus MM7 isolated from soil samples from Saudi Arabia. Life Sciences Journal 10(4):1884–1891.
Anderson, A.J. and Dawes, E.A., 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiology Review 54(4):450–472. 10.1128/mr.54.4.450-472.1990
Ashraf, S., Ali, S. and Ul-Haq, I., 2015. Acidic pre-treatment of sug-arcane molasses for citric acid production by Aspergillus niger NG-4. International Journal of Current Microbiology and Applied Sciences 4(6):584–595.
Baiano, A., 2014. Recovery of biomolecules from food wastes: a review. Molecules 19(9):14821–14842. 10.3390/molecules190914821
Bandaiphet, C. and Prasertsan, P., 2006. Effect of aeration and agitation rates and scale-up on oxygen transfer coefficient, kLa in exopolysaccharide production from Enterobacter cloacae WD7. Carbohydrate Polymers 66(2):216–228. 10.1016/j.carbpol.2006.03.004
Belal, E.B., 2013. Production ofpoly-β-hydroxybutyric acid (PHB) by Rhizobium elti and Pseudomonas stutzeri. Current Research Journal of Biological Sciences 5(6):273–284. 10.19026/crjbs.5.5429
Bhagowati, P., Pradhan, S., Dash, H.R. and Das, S., 2015. Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillus spp. Biosciences Biotechnology and Biochemistry 79(9):1454–1463. 10.1080/09168451.2015.1034651
Blunt, W., Levin, D.B. and Cicek, N., 2018. Bioreactor operating strategies for improved polyhydroxyalkanoate (PHA) productivity. Polymers 10(11):1197. 10.3390/polym10111197
Bouguedoura, N., Bennaceur, M., Babahani, S. and Benziouche, S.E., 2015. Date palm status and perspective in Algeria. In: Elkhayri, J.A., Jain, S.M. and Johnson, D.V. (eds.) Date palm genetic resources and utilization, volume 1 Africa and the Americas. Springer Dordrecht, pp. 125–168.
Chandrasekaran, M. and Bahkali, A.H., 2013. Valorization of date palm (Phoenix dactylifera) fruit processing by-products and wastes using bioprocess technology–review. Saudi Journal of Biological Sciences 20(2):105–120. 10.1016/j.sjbs.2012.12.004
Chniti, S., Jemni, M., Bentahar, I., Shariati, M., Djelal, H., Amrane, A. and Hassouna, M., 2017. By-products of dates: optimization of the extraction of juice using response surface methodology and ethanol production. Journal of Microbiology Biotechnology and Food Sciences 7(2):204–208. 10.15414/jmbfs.2017.7.2.204-208
El-Nagga, E.A. and Abd El-tawab, Y.A., 2012. Compositional characteristics of date syrup extracted by different methods in some fermented dairy products. Annals of Agricultural Sciences 57(1):29–36. 10.1016/j.aoas.2012.03.007
Elsayed, N.S., Aboulwafa, M., Aboshanab, K. and Hassouna, N., 2013. PHB production in Azomonas, Acinteobacter and Bacillus species: isolation, screening and identification. Archives of Clinical Microbiology 4(5):5025–5035. 10.3823/271
Gabr, G.A., 2018. Isolation and identification of bacterial strains able to biopolymer polyhydroxybutyrate (PHB) production from soil of Al-Kharj probes, Saudi Arabia. Journal of Pharmaceutical Research International 21(6):1–11. 10.9734/jpri/2018/39532
Getachew, A. and Woldesenbet, F., 2016. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Research Notes 9:509. 10.1186/s13104-016-2321-y
Gomaa, E.Z., 2014. Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coligrown on cane molasses fortified with ethanol. Brazilian Archives of Biology and Technology 57(1):145–154. 10.1590/S1516-89132014000100020
Gurieff, N. and Lant, P., 2007. Comparative life cycle analysis and financial analysis of polyhydroxyalkanoate production in mixed culture. Bioresource Technology 98(17): 3393–3403. 10.1016/j.biortech.2006.10.046
Gusakov, A.V., Kondratyeva, E.G. and Sinitsyn, A.P., 2011. Comparison of two methods for the determination of reducing sugars in the determination of carbohydrase activities. Journal of Analytical Chemistry 2011:283658. 10.1155/2011/283658
Hungund, B., Shyama, V.S., Patwardhan P. and Saleh, A.M., 2013. Production of polyhydroxyalkanoate from Paenibacillus durus BV-1 isolated from oil mill soil. Journal of Microbial and Biochemical Technology 5(1):13–17. 10.4172/1948-5948.1000092
Karr, D.B., Waters, J.K. and Emerich D.W., 1983. Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Applied Environmental Microbiology 46(6): 1339–1344. 10.1128/aem.46.6.1339-1344.1983
Keshavarz, T. and Roy, I., 2010. Polyhydroxyalkanoates bioplastics with a green agenda. Current Opinion in Microbiology 13(3):321–326. 10.1016/j.mib.2010.02.006
Kritika, S., Pragya, R., Nandini, P. and Priti, S., 2016. Optimization of PHB (poly-hydroxybutyrate) synthesis by Serratia sp. isolated from soil. International Journal of Current Microbiology and Applied Sciences 5(6):665–673. 10.20546/ijcmas.2016.506.072
Kundu, S., Panda, T., Majumdar, S.K., Guh, B. and Bandyopadhyay, K.K., 1984. Pretreatment of Indian cane molasses for increased production of citric acid. Biotechnology and Bioengineering 26(9):1114–1121. 10.1002/bit.260260915
Majzoobi, M., Karambakhsh, G., Golmakani, M.T., Mesbahi, G. and Farahnaky, A., 2020. Effects of level and particle size of date fruit press cake on batter rheological properties and physical and nutritional properties of cake. Journal of Agricultural Science and Technology 22(1):121–133. Available at: http://jast.modares.ac.ir/article-23-25558-en.html
Mantzouridou, F., Roukas, T. and Kekidou, P., 2002. Effect of the aeration rate and agitation speed on β-carotene production and morphology of Blakeslea trispora in a stirred tank reactor: mathematical modeling. Biochemical Engineering Journal 10(2): 123–135. 10.1016/s1369-703x(01)00166-8
McAdam, B., Fournet, M.B., McDonald, P. and Mojicevic, M., 2020. Production of polyhydroxybutyrate (PHB) and factors impacting its chemical and mechanical characteristics. Polymers 12(12): 1–20. 10.3390/polym12122908
Mohapatra, S., Maity, S., Dash, H.R., Das, S., Pattnaik, S., Rath C.C. and Samantaray, D., 2017. Bacillus and biopolymer: prospects and challenges. Biochemistry and Biophysics Reports 12: 206–213. 10.1016/j.bbrep.2017.10.001
Mohapatra, S., Pattnaik, S., Maity, S., Sharma, S., Akhtar, J., Pati, S., Samantaray, D.P. and Varma, A., 2020. Comparative analysis of PHAs production by Bacillus megaterium OUAT 016 under submerged and solid-state fermentation. Saudi Journal of Biological Sciences 27(5):1242–1250. 10.1016/j.sjbs.2020.02.001
Mohd Zahari, M.A.K., Ariffin, H., Mokhtar, M., Salihon, J., Shirai, Y. and Hassan, M.A., 2012. Factors affecting poly (3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG 5 2 2 3 8 T). Journal of Biomedicine and Biotechnology 2012:125865. 10.1155/2012/125865
Mostafa, Y.S., Alrumman, S.A., Alamri, S.A., Otaif, K.A., Mostafa, M.S. and Alfaify A.M., 2020. Bioplastic (poly-3-hydroxybutyrate) production by the marine bacterium Pseudodonghicola xiamenensis through date syrup valorization and structural assessment of the biopolymer. Scientific Reports 10(1):8815. 10.1038/s41598-020-65858-5
Naheed, N., Jamil, N., Hasnain, S. and Abbas, G., 2012. Biosynthesis of polyhydroxybutyrate in Enterobacter sp. SEL2 and the bacterium Enterobacteriaceae sp. PFW1 using sugar cane molasses as a carrier. African Journal of Biotechnology 11(16):3321–3332. 10.5897/AJB11.1405
Nancib, A., Nancib, N., Boubendir, A. and Boudrant, J., 2015. The use of date waste for lactic acid production by a fed-batch culture using Lactobacillus casei subsp. rhamnosus. Brazilian Journal of Microbiology 46(3):893–902. 10.1590/S1517-838246320131067
Narayanan, M., Kumarasamy, S., Ranganathan, M., Kandasamy, S., Kandasamy, G. and Gnanavel, K., 2020. Enzyme and metabolites attained in degradation of chemical pesticides ß-Cypermethrin by Bacillus cereus. Materials Today: Proceedings 33(7): 3640–3645. 10.1016/j.matpr.2020.05.722
Omar, S., Rayes, A., Eqaab, A., Vob, I. and Seinbüchel, A., 2001. Optimization of cell growth and poly (3-hydroxybutyrate) accumulation on date syrup by a Bacillus megaterium strain. Biotechnology Letters 23(14):1119–1123. 10.1023/a:1010559800535
Patel, N., Patel, P. and Desai, R., 2017. Detection and characterization of PHB (polyhydroxybutyrate) producers halophilic bacteria isolated from marine water sample of Valsad District. International Journal of Pharma and Bio Sciences 8(3): 1100–1108. 10.22376/ijpbs.2017.8.3.b1100-1108
Sen, K.Y., Hussin, M.H. and Baidurah, S., 2019. Biosynthesis of poly (3-hydroxybutyrate) (PHB) by Cupriavidus necator from various molasses pretreated as a carbon source. Biocatalysis and Agricultural Biotechnology 17:51–59. 10.1016/j.bcab.2018.11.006
Sharma, L., Singh, A.K., Panda, B. and Mallick, N., 2007. Process optimization for poly β-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology. Bioresource Technology 98(5):987–993. 10.1016/j.biortech.2006.04.016
Singh, G., Kumari, A., Mittal, A., Yadav, A. and Aggarwal, N.K., 2013. Poly β-hydroxybutyrate production by Bacillus subtilis NG220 using sugar industry waste water. BioMedResearch International 2013: 952641. 10.1155/2013/952641
Tallon, P., Magajna, B., Lofranco, C. and Leung, K.T., 2005. Microbial indicators of faecal contamination in water: a current perspective. Water, Air and Soil Pollution 166:139–166. 10.1007/s11270-005-7905-4
Thapa, C., Shakya, P., Shrestha, R., Pal, S. and Manandhar, P., 2018. Isolation of polyhydroxybutyrate (PHB) producing bacteria, optimization of culture conditions for PHB production, extraction and characterization of PHB. Nepal Journal of Biotechnology 6(1):62–68. 10.3126/njb.v6i1.22339
Vu, D.H., Wainaina, S., Taherzadeh, M.J., Åkesson, D. and Ferreira, J.A., 2021. Production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using food waste acidogenic fermentation-derived volatile fatty acids. Bioengineered 12(1):2480–2498. 10.1080/21655979.2021.1935524
Werlang, E.B., Moraes, L.B., Muller, M.V.G., Julich, J., Corbellini, V.A., Neves, F.D.F., de Souza, D., Benitez, L.B. and Schneider, R.D.C.D., 2021. Polyhydroxybutyrate (PHB) production via bio-conversion using Bacillus pumilus in liquid phase cultivation of the biomass of Arthrospira platensis hydrolysate as a carbon source. Waste and Biomass Valorization 12:3245–3255. 10.1007/s12649-020-01213-z
Adwitiya, P., Ashwini, P., Avinash, A.K., Badri, R., Kajal, D., Vomsi, P. and Srividya, S., 2009. Mutagenesis of Bacillus thuringiensis IAM 12077 to increase the production of poly backslash beta-hydroxybutyrate (PHB). Turkish Journal of Biology 33(3): 225–223. 10.3906/biy-0808-10
Aljuraifani, A.A., Berekaa, M.M. and Ghazwani, A.A., 2019. Bacterial biopolymer (polyhydroxyalkanoate) production from low-cost sustainable sources. Microbiology Open 8(6):e00755. 10.1002/mbo3.755
Ahmad, A., Naqvi, A., Jaskani, M.J., Waseem, M., Ali, E., Khan, I.A., Manzoor, M.F., Siddeeg, A. and Aadil, R.M., 2021. Efficient utilization of date palm waste for the bioethanol production through Saccharomyces cerevisiae strain. Food Sciences & Nutrition 9(4):2066–2074. 10.1002/fsn3.2175
Aly, M.M., Albureikan, M., El Rabey, H. and Kabli, S.A., 2013. Effects of culture conditions on growth and poly–beta-hydroxybutyric acid production by Bacillus cereus MM7 isolated from soil samples from Saudi Arabia. Life Sciences Journal 10(4):1884–1891.
Anderson, A.J. and Dawes, E.A., 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiology Review 54(4):450–472. 10.1128/mr.54.4.450-472.1990
Ashraf, S., Ali, S. and Ul-Haq, I., 2015. Acidic pre-treatment of sug-arcane molasses for citric acid production by Aspergillus niger NG-4. International Journal of Current Microbiology and Applied Sciences 4(6):584–595.
Baiano, A., 2014. Recovery of biomolecules from food wastes: a review. Molecules 19(9):14821–14842. 10.3390/molecules190914821
Bandaiphet, C. and Prasertsan, P., 2006. Effect of aeration and agitation rates and scale-up on oxygen transfer coefficient, kLa in exopolysaccharide production from Enterobacter cloacae WD7. Carbohydrate Polymers 66(2):216–228. 10.1016/j.carbpol.2006.03.004
Belal, E.B., 2013. Production ofpoly-β-hydroxybutyric acid (PHB) by Rhizobium elti and Pseudomonas stutzeri. Current Research Journal of Biological Sciences 5(6):273–284. 10.19026/crjbs.5.5429
Bhagowati, P., Pradhan, S., Dash, H.R. and Das, S., 2015. Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillus spp. Biosciences Biotechnology and Biochemistry 79(9):1454–1463. 10.1080/09168451.2015.1034651
Blunt, W., Levin, D.B. and Cicek, N., 2018. Bioreactor operating strategies for improved polyhydroxyalkanoate (PHA) productivity. Polymers 10(11):1197. 10.3390/polym10111197
Bouguedoura, N., Bennaceur, M., Babahani, S. and Benziouche, S.E., 2015. Date palm status and perspective in Algeria. In: Elkhayri, J.A., Jain, S.M. and Johnson, D.V. (eds.) Date palm genetic resources and utilization, volume 1 Africa and the Americas. Springer Dordrecht, pp. 125–168.
Chandrasekaran, M. and Bahkali, A.H., 2013. Valorization of date palm (Phoenix dactylifera) fruit processing by-products and wastes using bioprocess technology–review. Saudi Journal of Biological Sciences 20(2):105–120. 10.1016/j.sjbs.2012.12.004
Chniti, S., Jemni, M., Bentahar, I., Shariati, M., Djelal, H., Amrane, A. and Hassouna, M., 2017. By-products of dates: optimization of the extraction of juice using response surface methodology and ethanol production. Journal of Microbiology Biotechnology and Food Sciences 7(2):204–208. 10.15414/jmbfs.2017.7.2.204-208
El-Nagga, E.A. and Abd El-tawab, Y.A., 2012. Compositional characteristics of date syrup extracted by different methods in some fermented dairy products. Annals of Agricultural Sciences 57(1):29–36. 10.1016/j.aoas.2012.03.007
Elsayed, N.S., Aboulwafa, M., Aboshanab, K. and Hassouna, N., 2013. PHB production in Azomonas, Acinteobacter and Bacillus species: isolation, screening and identification. Archives of Clinical Microbiology 4(5):5025–5035. 10.3823/271
Gabr, G.A., 2018. Isolation and identification of bacterial strains able to biopolymer polyhydroxybutyrate (PHB) production from soil of Al-Kharj probes, Saudi Arabia. Journal of Pharmaceutical Research International 21(6):1–11. 10.9734/jpri/2018/39532
Getachew, A. and Woldesenbet, F., 2016. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Research Notes 9:509. 10.1186/s13104-016-2321-y
Gomaa, E.Z., 2014. Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coligrown on cane molasses fortified with ethanol. Brazilian Archives of Biology and Technology 57(1):145–154. 10.1590/S1516-89132014000100020
Gurieff, N. and Lant, P., 2007. Comparative life cycle analysis and financial analysis of polyhydroxyalkanoate production in mixed culture. Bioresource Technology 98(17): 3393–3403. 10.1016/j.biortech.2006.10.046
Gusakov, A.V., Kondratyeva, E.G. and Sinitsyn, A.P., 2011. Comparison of two methods for the determination of reducing sugars in the determination of carbohydrase activities. Journal of Analytical Chemistry 2011:283658. 10.1155/2011/283658
Hungund, B., Shyama, V.S., Patwardhan P. and Saleh, A.M., 2013. Production of polyhydroxyalkanoate from Paenibacillus durus BV-1 isolated from oil mill soil. Journal of Microbial and Biochemical Technology 5(1):13–17. 10.4172/1948-5948.1000092
Karr, D.B., Waters, J.K. and Emerich D.W., 1983. Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Applied Environmental Microbiology 46(6): 1339–1344. 10.1128/aem.46.6.1339-1344.1983
Keshavarz, T. and Roy, I., 2010. Polyhydroxyalkanoates bioplastics with a green agenda. Current Opinion in Microbiology 13(3):321–326. 10.1016/j.mib.2010.02.006
Kritika, S., Pragya, R., Nandini, P. and Priti, S., 2016. Optimization of PHB (poly-hydroxybutyrate) synthesis by Serratia sp. isolated from soil. International Journal of Current Microbiology and Applied Sciences 5(6):665–673. 10.20546/ijcmas.2016.506.072
Kundu, S., Panda, T., Majumdar, S.K., Guh, B. and Bandyopadhyay, K.K., 1984. Pretreatment of Indian cane molasses for increased production of citric acid. Biotechnology and Bioengineering 26(9):1114–1121. 10.1002/bit.260260915
Majzoobi, M., Karambakhsh, G., Golmakani, M.T., Mesbahi, G. and Farahnaky, A., 2020. Effects of level and particle size of date fruit press cake on batter rheological properties and physical and nutritional properties of cake. Journal of Agricultural Science and Technology 22(1):121–133. Available at: http://jast.modares.ac.ir/article-23-25558-en.html
Mantzouridou, F., Roukas, T. and Kekidou, P., 2002. Effect of the aeration rate and agitation speed on β-carotene production and morphology of Blakeslea trispora in a stirred tank reactor: mathematical modeling. Biochemical Engineering Journal 10(2): 123–135. 10.1016/s1369-703x(01)00166-8
McAdam, B., Fournet, M.B., McDonald, P. and Mojicevic, M., 2020. Production of polyhydroxybutyrate (PHB) and factors impacting its chemical and mechanical characteristics. Polymers 12(12): 1–20. 10.3390/polym12122908
Mohapatra, S., Maity, S., Dash, H.R., Das, S., Pattnaik, S., Rath C.C. and Samantaray, D., 2017. Bacillus and biopolymer: prospects and challenges. Biochemistry and Biophysics Reports 12: 206–213. 10.1016/j.bbrep.2017.10.001
Mohapatra, S., Pattnaik, S., Maity, S., Sharma, S., Akhtar, J., Pati, S., Samantaray, D.P. and Varma, A., 2020. Comparative analysis of PHAs production by Bacillus megaterium OUAT 016 under submerged and solid-state fermentation. Saudi Journal of Biological Sciences 27(5):1242–1250. 10.1016/j.sjbs.2020.02.001
Mohd Zahari, M.A.K., Ariffin, H., Mokhtar, M., Salihon, J., Shirai, Y. and Hassan, M.A., 2012. Factors affecting poly (3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG 5 2 2 3 8 T). Journal of Biomedicine and Biotechnology 2012:125865. 10.1155/2012/125865
Mostafa, Y.S., Alrumman, S.A., Alamri, S.A., Otaif, K.A., Mostafa, M.S. and Alfaify A.M., 2020. Bioplastic (poly-3-hydroxybutyrate) production by the marine bacterium Pseudodonghicola xiamenensis through date syrup valorization and structural assessment of the biopolymer. Scientific Reports 10(1):8815. 10.1038/s41598-020-65858-5
Naheed, N., Jamil, N., Hasnain, S. and Abbas, G., 2012. Biosynthesis of polyhydroxybutyrate in Enterobacter sp. SEL2 and the bacterium Enterobacteriaceae sp. PFW1 using sugar cane molasses as a carrier. African Journal of Biotechnology 11(16):3321–3332. 10.5897/AJB11.1405
Nancib, A., Nancib, N., Boubendir, A. and Boudrant, J., 2015. The use of date waste for lactic acid production by a fed-batch culture using Lactobacillus casei subsp. rhamnosus. Brazilian Journal of Microbiology 46(3):893–902. 10.1590/S1517-838246320131067
Narayanan, M., Kumarasamy, S., Ranganathan, M., Kandasamy, S., Kandasamy, G. and Gnanavel, K., 2020. Enzyme and metabolites attained in degradation of chemical pesticides ß-Cypermethrin by Bacillus cereus. Materials Today: Proceedings 33(7): 3640–3645. 10.1016/j.matpr.2020.05.722
Omar, S., Rayes, A., Eqaab, A., Vob, I. and Seinbüchel, A., 2001. Optimization of cell growth and poly (3-hydroxybutyrate) accumulation on date syrup by a Bacillus megaterium strain. Biotechnology Letters 23(14):1119–1123. 10.1023/a:1010559800535
Patel, N., Patel, P. and Desai, R., 2017. Detection and characterization of PHB (polyhydroxybutyrate) producers halophilic bacteria isolated from marine water sample of Valsad District. International Journal of Pharma and Bio Sciences 8(3): 1100–1108. 10.22376/ijpbs.2017.8.3.b1100-1108
Sen, K.Y., Hussin, M.H. and Baidurah, S., 2019. Biosynthesis of poly (3-hydroxybutyrate) (PHB) by Cupriavidus necator from various molasses pretreated as a carbon source. Biocatalysis and Agricultural Biotechnology 17:51–59. 10.1016/j.bcab.2018.11.006
Sharma, L., Singh, A.K., Panda, B. and Mallick, N., 2007. Process optimization for poly β-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology. Bioresource Technology 98(5):987–993. 10.1016/j.biortech.2006.04.016
Singh, G., Kumari, A., Mittal, A., Yadav, A. and Aggarwal, N.K., 2013. Poly β-hydroxybutyrate production by Bacillus subtilis NG220 using sugar industry waste water. BioMedResearch International 2013: 952641. 10.1155/2013/952641
Tallon, P., Magajna, B., Lofranco, C. and Leung, K.T., 2005. Microbial indicators of faecal contamination in water: a current perspective. Water, Air and Soil Pollution 166:139–166. 10.1007/s11270-005-7905-4
Thapa, C., Shakya, P., Shrestha, R., Pal, S. and Manandhar, P., 2018. Isolation of polyhydroxybutyrate (PHB) producing bacteria, optimization of culture conditions for PHB production, extraction and characterization of PHB. Nepal Journal of Biotechnology 6(1):62–68. 10.3126/njb.v6i1.22339
Vu, D.H., Wainaina, S., Taherzadeh, M.J., Åkesson, D. and Ferreira, J.A., 2021. Production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using food waste acidogenic fermentation-derived volatile fatty acids. Bioengineered 12(1):2480–2498. 10.1080/21655979.2021.1935524
Werlang, E.B., Moraes, L.B., Muller, M.V.G., Julich, J., Corbellini, V.A., Neves, F.D.F., de Souza, D., Benitez, L.B. and Schneider, R.D.C.D., 2021. Polyhydroxybutyrate (PHB) production via bio-conversion using Bacillus pumilus in liquid phase cultivation of the biomass of Arthrospira platensis hydrolysate as a carbon source. Waste and Biomass Valorization 12:3245–3255. 10.1007/s12649-020-01213-z
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Aug 23, 2022
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Chekroud, Z., Djerrab, L., Rouabhia, A., Dems, M. A., Atailia, I., Djazy, F., & Smadi, M. A. (2022). Valorisation of date fruits by-products for the production of biopolymer polyhydroxybutyrate (PHB) using the bacterial strain Bacillus paramycoides. Italian Journal of Food Science, 34(3), 48-58. https://doi.org/10.15586/ijfs.v34i3.2236
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How to Cite
Chekroud, Z., Djerrab, L., Rouabhia, A., Dems, M. A., Atailia, I., Djazy, F., & Smadi, M. A. (2022). Valorisation of date fruits by-products for the production of biopolymer polyhydroxybutyrate (PHB) using the bacterial strain Bacillus paramycoides. Italian Journal of Food Science, 34(3), 48-58. https://doi.org/10.15586/ijfs.v34i3.2236