Ultrasound-assisted extraction of Gac (Momordica cochinchinensis Spreng.) leaves: Effect of maturity stage on phytochemicals and carbohydrate-hydrolyzing enzymes inhibitory activity

Main Article Content

Thi Minh Chau Nguyen
Mohsen Gavahian
Pi-Jen Tsai

Keywords

Abstract

Although phytochemical contents of Gac fruit have been extensively analyzed, information about the bioactive compounds and valorization of Gac leaves is limited. In this study, Gac (Momordica cochinchinensis Spreng.) leaves at different maturity stages (young: YL, mature: ML and old: OL leaves) were extracted during a 20 min of 150-W sonication process. Color, phytochemicals, antioxidant activity, and inhibitory effects against carbohydrate-hydrolyzing enzymes were assessed by colorimetric, high-performance liquid chromatography, and spectrophotometric methods, respectively. Results indicated a decrease in L* (lightness) and an increase in a* (greenness–redness) during maturation of leaves. The YL extract had the highest contents of phytochemicals with 4897.01 (mg gallic acid equivalent [GAE] per 100 gram dried weight [DW]), total phenolics, 592.81 (mg querce-tin [QE]/100 g DW), total flavonoids, 34.77% ?-amylase inhibitory activity, and 40.21% ?-glucosidase inhibitory activity. Myricetin (43%), vitexin (22%), and esculetin (11%) were the major bioactive compounds detected in YL extract. Also, the superoxide dismutase (SOD)-like capacity of the extract decreased from 11,599.96 to 3,999.63 U/g DW during the transformation of YL to OL. Extract of Gac leaves was found to be a potential ingredient for food preservation and supplementation that could reduce postprandial hyperglycemia.

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References

Abdulqader, A., Ali, F., Ismail, A. and Esa, N., 2018. Gac (Momordica cochinchinensis Spreng.) fruit and its potentiality and superiority in health benefits. Journal of Contemporary Medical Sciences 4(4). 179–186.
Abeysekera, W.P.K.M., Arachchige, S.P.G., Abeysekera, W.K.S.M., Ratnasooriya, W.D. and Medawatta, H.M.U.I. (2019). Antioxidant and glycemic regulatory properties potential of different maturity stages of leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) in vitro. Evidence-Based Complementary and Alternative Medicine, 2019. https://doi. org/10.1155/2019/2693795
Adekola, K.A., Salleh, A.B., Zaidan, U.H., Azlan, A., Chiavaro, E., Paciulli, M., et al. (2017). Total phenolic content, antioxidative and antidiabetic properties of coconut (Cocos nucífera L.) testa and selected bean seed coats. Italian Journal of Food Science 29(4). 741–753. https://doi.org/10.14674/IJFS-941
Ademosun, M.T., Omoba, O.S. and Olagunju, A.I., 2020. Antioxidant properties, glycemic indices, and carbohydrate hydrolyzing enzymes activities of formulated ginger-based fruit drinks. Journal of Food Biochemistry, e13324. https://doi. org/10.1111/jfbc.13324
AL-Ishaq, R.K., Abotaleb, M., Kubatka, P., Kajo, K. and Büsselberg, D., 2019. Flavonoids and their anti-diabetic effects: cellular mechanisms and effects to improve blood sugar levels. Biomolecules 9(9): 430. https://doi.org/10.3390/biom9090430
Angmo, P., Chorol, S., Namgail, D., Chaurasia, O. and Stobdan, T. 2019. Effect of maturation on phenolics and flavonoids content of greenhouse-grown beet leaf. Pharmacognosy Journal 11(5): 1010–1013. https://doi.org/10.5530/pj.2019.11.159
Assefa, S.T., Yang, E.-Y., Chae, S.-Y., Song, M., Lee, J., Cho, M.-C., et al., S. 2020. Alpha glucosidase inhibitory activities of plants with focus on common vegetables. Plants 9(1): 2. https://doi.org/10.3390/plants9010002
Cheng, C.-W., Chen, L.-Y., Chou, C.-W. and Liang, J.-Y. 2015. Investigations of riboflavin photolysis via coloured light in the nitro blue tetrazolium assay for superoxide dismutase activity. Journal of Photochemistry and Photobiology B: Biology 148: 262–267. https://doi.org/10.1016/j.jphotobiol.2015.04.028
Chuyen, H.V., Nguyen, M.H., Roach, P.D., Golding, J.B. and Parks,  S.E., 2014. Gac fruit (Momordica cochinchinensis Spreng.): a rich source of bioactive compounds and its poten-tial health benefits. International Journal of Food Science & Technology 50(3): 567–577. https://doi.org/10.1111/ijfs.12721
Chuyen, H.V., Roach, P.D., Golding, J.B., Parks, S.E. and Nguyen,  M.H., 2020. Ultrasound-assisted extraction of GAC peel: an optimization of extraction conditions for recovering carotenoids and antioxidant capacity. Processes 8(1): 8. https:// doi.org/10.3390/pr8010008
Dobrin?i?, A., Repaji?, M., Garofuli?, I.E., Tu?en, L., Dragovi?-Uzelac, V. and Levaj, B., 2020. Comparison of different extraction methods for the recovery of olive leaves polyphenols. Processes 8(9): 1008. https://doi.org/10.3390/pr8091008
dos Santos Pereira, E., Vinholes, J.R., Camargo, T.M., Nora, F.R., Crizel, R.L., Chaves, F., et al. 2020. Characterization of araçá fruits (Psidium cattleianum Sabine): phenolic composition, antioxidant activity and inhibition of ?-amylase and ?-gluco-sidase. Food Bioscience 37: 100665. https://doi.org/10.1016/j. fbio.2020.100665
Guglielmetti, A., D’ignoti, V., Ghirardello, D., Belviso, S. and Zeppa,  G., 2017. Optimisation of ultrasound and microwave-assisted extraction of caffeoylquinic acids and caffeine from coffee silverskin using response surface methodology. Italian Journal of Food Science 29(3): 409–423. http://orcid.org/0000-0002-0397-6880. https://doi.org/10.14674/IJFS-727
??çimen, E.M. and Hayta, M.E.H.M.E.T. 2018. Optimisation of ultrasound-assisted extraction of rice bran proteins: effects on antioxidant and antiproliferative properties. Quality Assurance and Safety of Crops & Foods 10(2): 165–174. https://doi. org/10.3920/QAS2017.1186
Karadag, A., Pelvan, E., Dogan, K., Celik, N., Ozturk, D., Akal?n, K., et al. 2019. Optimisation of green tea polysaccharides by ultrasound-assisted extraction and their in vitro antidiabetic activities. Quality Assurance and Safety of Crops & Foods 11(5): 479–490. https://doi.org/10.3920/QAS2019.1579
Kha, T.C., Nguyen, M.H., Roach, P.D., Parks, S.E. and Stathopoulos, C., 2013. Gac fruit: nutrient and phytochemical composition, and options for processing. Food Reviews International 29(1): 92–106. https://doi.org/10.1080/87559129.2012.692141
Mogole, L., Omwoyo, W. and Mtunzi, F., 2020. Phytochemical screen-ing, anti-oxidant activity and ?-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves. Heliyon 6(8): e04736. https://doi.org/10.1016/j.heliyon.2020.e04736
Moreira, S.A., Alexandre, E.M., Pintado, M. and Saraiva, J.A., 2019. Effect of emergent non-thermal extraction technologies on bio-active individual compounds profile from different plant mate-rials. Food Research International 115: 177–190. https://doi. org/10.1016/j.foodres.2018.08.046
Munekata, P.E., Alcántara, C., Žug?i?, T., Abdelkebir, R., Collado,  M.C., García-Pérez, J.V., et al. 2020. Impact of ultrasound-assisted extraction and solvent composition on bio-active compounds and in vitro biological activities of thyme and rosemary. Food Research International 134: 109242. https://doi. org/10.1016/j.foodres.2020.109242
Nagarani, G., Abirami, A. and Siddhuraju, P., 2014. A comparative study on antioxidant potentials, inhibitory activities against key enzymes related to metabolic syndrome, and anti-inflammatory activity of leaf extract from different Momordica species. Food Science and Human Wellness 3(1): 36–46. https://doi. org/10.1016/j.fshw.2014.02.003
Ng, Z.X. and See, A.N., 2019. Effect of in vitro digestion on the total polyphenol and flavonoid, antioxidant activity and carbohydrate hydrolyzing enzymes inhibitory potential of selected functional plant-based foods. Journal of Food Processing and Preservation 43(4): e13903. https://doi.org/10.1111/jfpp.13903
Ngamsuk, S., Huang, T.-C. and Hsu, J.-L., 2019. Determination of phenolic compounds, procyanidins, and antioxidant activity in processed Coffea arabica L. leaves. Foods 8(9): 389. https://doi. org/10.3390/foods8090389
Nguyen, V., Nguyen, M., Tran, Q., Thinh, P., Bui, L., Le, T., et al. 2020. Effect of extraction solvent on total polyphenol content, total flavonoid content, and antioxidant activity of soursop seeds (Annona muricata L.). IOP Conference Series Materials Science and Engineering (MS&E) 736(2): 022063. https://doi. org/10.1088/1757-899X/736/2/022063
Özcan, K., 2020. Antibacterial, antioxidant and enzyme inhibition activity capacities of Doronicum macrolepis (FREYN & SINT): an endemic plant from Turkey. Saudi Pharmaceutical Journal 28(1): 95–100. https://doi.org/10.1016/j.jsps.2019.11.010
Patsilinakos, A., Ragno, R., Carradori, S., Petralito, S. and Cesa, S., 2018. Carotenoid content of Goji berries: CIELAB, HPLC-DAD analyses and quantitative correlation. Food Chemistry 268: 49–56. https://doi.org/10.1016/j.foodchem.2018.06.013
Proença, C., Freitas, M., Ribeiro, D., Oliveira, E.F., Sousa, J.L., Tomé, S.M., et al. 2017. ?-Glucosidase inhibition by flavonoids: an in vitro and in silico structure–activity relationship study. Journal of Enzyme Inhibition and Medicinal Chemistry 32(1): 1216–1228. https://doi.org/10.1080/14756366.2017.1368503
Roukas, T. and Kotzekidou, P., 2020. Pomegranate peel waste: a new substrate for citric acid production by Aspergillus niger in solid-state fermentation under non-aseptic conditions. Environmental Science and Pollution Research 27: 13105– 13113. https://doi.org/10.1007/s11356-020-07928-9
Shimada, A., Ueno, H., Inagaki, M. and Yoshimitsu, H., 2020. Comparative studies of the biological activities of selected herbal extracts and phenolic compounds isolated from Rosa gallica. Zeitschrift für Naturforschung C 75(1–2): 31–39. https://doi. org/10.1515/znc-2019-0117
Shori, A.B., 2020. Proteolytic activity, antioxidant, and ?-amylase inhibitory activity of yogurt enriched with coriander and cumin seeds. LWT—Food Science and Technologyv 138: 109912. https://doi.org/10.1016/j.lwt.2020.109912
Singleton, V.L. and Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3): 144–158.
Stephenie, S., Chang, Y.P., Gnanasekaran, A., Esa, N.M. and Gnanaraj, C., 2020. An insight on superoxide dismutase (SOD) from plants for mammalian health enhancement. Journal of Functional Foods 68: 103917. https://doi.org/10.1016/j. jff.2020.103917
Surin, S., You, S., Seesuriyachan, P., Muangrat, R., Wangtueai,  S., Jambrak, A.R., et al. 2020. Optimization of ultrasonic-assisted extraction of polysaccharides from purple glutinous rice bran (Oryza sativa L.) and their antioxidant activities. Scientific Reports 10(1): 1–10. https://doi.org/10.1038/ s41598-020-67266-1
Tan, Y., Chang, S.K. and Zhang, Y., 2017. Comparison of ?-amylase, ?-glucosidase and lipase inhibitory activity of the phenolic sub-stances in two black legumes of different genera. Food Chemistry 214: 259–268. https://doi.org/10.1016/j.foodchem.2016.06.100
Tian, J., Muhammad, S., Chen, A., Chen, P., Wang, J., Yang, C., et al. 2019. An experimental study exploring the influencing factors for ultrasonic-assisted extraction of flavonoid compounds from leaves of Amorpha fruticosa L. Journal of Forestry Research 30(5): 1735–1741. https://doi.org/10.1007/s11676-019-00931-y
Veiga, M., Costa, E.M., Silva, S. and Pintado, M., 2020. Impact of plant extracts upon human health: a review. Critical Reviews in Food Science and Nutrition 60(5): 873–886. https://doi.org/10.1 080/10408398.2018.1540969
Yu, J.S., Roh, H.-S., Lee, S., Jung, K., Baek, K.-H. and Kim, K.H., 2017. Antiproliferative effect of Momordica cochinchinensis seeds on human lung cancer cells and isolation of the major constituents. Revista Brasileira de Farmacognosia 27(3): 329–333. https://doi.org/10.1016/j.bjp.2017.02.002