CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATES FROM TRADITIONAL DAIRY PRODUCTS OF SMALL-SCALE ALPINE FARMS
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Keywords
alpine small-scale dairies, dairy products, molecular characterisation, staphylococcal enterotoxins, Staphylococcus aureus
Abstract
This study investigated the prevalence of Staphylococcus aureus in raw milk dairy products handcrafted in traditional alpine small-scale farms, and characterised the enterotoxigenicity and resistance to methicillin. Among the analysed samples, 69% exceeded the international microbiological recommendations. The highest counts were observed for cheese or fatty products (~106 cfu/g). Conversely, lower contamination levels concerned raw milk and whey cheese (~102 cfu/g). A total of 163 S. aureus isolates were collected, and the prevalence of MRSA was low (1.7%) but not negligible. The finding of enterotoxins genes in 67% of the isolates is of concern for the public health.
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V. FILIPELLO, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via A. Bianchi 9, 25124 Brescia, Italy.
Virginia Filipello is a research scholar in the Department of Food Control of the Istituto Zooprofilattico della Lombardia e dell’Emilia Romagna (IZSLER). She received her Ph.D. in Veterinary Sciences at the University of Turin. Virginia’s research interests include Epidemiology of Foodborne Diseases, Molecular Biology, and Microbiology. Her work is currently focussed on the molecular epidemiology of Staphylococcus aureus and Listeria monocytogenes in Northern Italy.
M. TILOLA, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via A. Bianchi 9, 25124 Brescia, Italy.
Michela Tilola was born in 02/03/1979 and obtained a Master’s degree in Biotecnology at the Milan University in 2003. She works in the IZSLER since 2003 in the Food Microbiology Department. She has expertise on different molecular biology methods to detect and characterize viruses and bacteria from food, in particular PCR and Real-time PCR, bacterial genomic characterization with Automatic Ribotyping and PFGE (Pulsed Field Gel Electrophoresis) and in-vitro toxicology based on cell culture.
G. FINAZZI, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via A. Bianchi 9, 25124 Brescia, Italy.
Guido Finazzi was born in Palazzolo s/O, Italy, the 16th of June 1970. In 1994 he obtains his Master Degree in Veterinary Medicine, in 1998 a specialization in “Hygiene and technology of milk and dairy products, and in 2004 a Bachelor Degree in Veterinary Biotechnology. Since 2004, he works in the Department of “Food Microbiology” of IZSLER in Brescia on microbiological analysis according to EU Regulation and to international export criteria (US and other non-EU countries), characterization of typical food products, experimental trials to evaluate the behaviour of main pathogens during food processing and storage. He published 36 articles and more than 120 contributions to congresses and science dissemination.