Main Article Content
Apple, central composite design, flavonoids, HPLC, response surface methodology, ultrasonic extraction
The ultrasound-assisted extraction of flavonoids from apple samples was modelled using response surface methodology. A three-level-three-factor central composite design using the response surface methodology (RSM) was employed to optimise three extraction variables, including temperature, extraction time and ultrasonic power, for the achievement of the highest extraction yield of the flavonoids from lyophilised apple samples. The optimised extraction conditions were 44.61ºC, an extraction time of 26.90 min, and ultrasonic power 480 W. The experimental yield of flavonoids was 6.58 mg g-1 expressed as rutin equivalent, which was close to the predicted yield (6.69 mg g-1). Optimised extraction conditions were applied for the analysis of apple samples of six cultivars.
Canettieri E.V., de Moraes Rocha G.J., de Carvalho J.A. and de Almeida e Silva J.B. 2007. Optimization of acid hydrolysis from the hemicellulosic fraction of Eucalyptus grandis residue using response surface methodology. Bioresour. Technol. 98:422-428.
Ceymann M., Arrigoni E., Schärer H., Nising A.B. and Hurrell R.F. 2012. Identification of apples rich in health-promoting flavan-3-ols and phenolic acids by measuring the polyphenol profile. J. Food Comp. Anal. 26:128-135.
Chen W., Wang W.P., Zhang H.S. and Huang Q. 2012. Optimization of ultrasonic-assisted extraction of water-soluble polysaccharides from Boletus edulis mycelia using response surface methodology. Carbohydr. Poly. 87:614-619.
Cooke M.S., Evans M.D., Dizdaroglu M. and Lunec J. 2003. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J.17:1195-1214.
Duda-Chodak A., Tarko T. and Tuszynski T. 2011. Antioxidant activity of apples-an impact of maturity stage and fruit part. Acta Sci. Pol. Technol. Aliment. 10:443-454.
Duda-Chodak A., Tarko T., Satora P., Sroka P. and Tuszy?ski T. 2010. The profile of polyphenols and antioxidant properties of selected apple cultivars grown in Poland. J. Fruit Ornam. Plant Res. 18:39-50.
European Pharmacopoeia. 2010. vol. 1, 7th ed. Council of Europe, Strasbourg, France.
FAO. FAO Statistical Database. 2013. Retrieved from http://www.fao.org/faostat/en/#data/QC (Accessed 23-05-2017).
Gosch C., Halbwirth H. and Stich K. 2010. Phloridzin: biosynthesis, distribution and physiological relevance in plants. Phytochemistry. 71:838-843.
Kahle K., Kraus M. and Richling E. 2005. Polyphenol profiles of apple juices. Mol. Nutr. Food Res. 49:797-806.
Lata B., Przeradzka M. and Binkowska M. 2005. Great differences in antioxidant properties exist between 56 apple cultivars and vegetation seasons. J. Agric. Food Chem. 53:8970-8978.
Liaudanskas M., Viškelis P., Jakštas V., Raudonis R., Kviklys D., Milašius A. and Janulis V. 2014. Application of an optimized HPLC method for the detection of various phenolic compounds in apples from Lithuanian cultivars. J. Chem., 2014:Article ID 542121, 10 pages.
Madamanchi N.R., Vendrov A. and Runge M.S. 2005. Oxidative stress and vascular disease. Arterioscler. Thromb. Vasc. Biol. 25:29-38.
Marks S.C., Mullen W. and Crozier A. 2007. Flavonoid and chlorogenic acid profiles of English cider apples. J. Sci. Food Agr. 87:719?728.
Muralidhar R.V., Chirumamila R.R., Marchant R. and Nigam P. 2001. A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources. Biochem. Eng. J.9:17-23.
Pandey K.B. and Rizvi S.I. 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell Longev. 2:270-278.
Panzella L., Petriccione M., Rega P., Scortichini M., and Napolitano A. 2013. A reappraisal of traditional apple cultivars from Southern Italy as a rich source of phenols with superior antioxidant activity. Food Chem.140:672-679.
Pizzimenti S., Toaldo C., Pettazzoni P., Dianzani M.U. and Barrera G. 2010. The “two-faced” effects of reactive oxygen species and the lipid peroxidation product 4-hydroxyno-nenal in the hallmarks of cancer. Cancers. 2:338-363.
Price K.R., Prosser T., Richetin A.M. and Rhodes M.J.C. 1999. A comparison of the flavonol content and composition in dessert, cooking and cider-making apples; distribution within the fruit and effect of juicing. Food Chem. 66:489-494.
Radojkovic M., Zekovic Z., Jokic S., Vidovic S., Lepojevic Z. and Milosevic S. 2012. Optimization of solid-liquid extraction of antioxidants from black mulberry leaves by response surface methodology. Food Technol. Biotech. 50:167-176.
Reuter S., Gupta S.C., Chaturvedi M.M. and Aggarwal B.B. 2010. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic. Biol. Med. 49:1603-1616.
Sanoner P., Guyot S., Marnet N., Molle D. and Drilleau J.F. 1999. Polyphenol profiles of French cider apple varieties (Malus domestica sp.). J. Agric. Food Chem. 47:4847-4853.
Schieber A., Keller P. and Carle R. 2001. Determination of phenolic acids and flavonoids of apple and pear by high-performance liquid chromatography. J. Chromatogr. A. 910:265-273.
Tian Y., Xu Z., Zheng B., and Lo Y.M. 2013. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil. Ultrason. Sonochem. 20:202-208.
Urbonaviciute A., Jakštas V., Kornyšova O., Janulis V. and Maruška A. 2006. A. Capillary electrophoretic analysis of flavonoids in single-styled hawthorn (Crataegus monogyna Jacq.) ethanol extracts. J. Chromatogr. A. 1112:339-344.
van der Sluis A.A., Dekker M.A., Jager A. and Jongen W.M.F. 2001. Activity and concentration of polyphenolic antioxidants in apple: effect of cultivar, harvest year, and storage conditions. J. Agric. Food Chem. 48:3606-3613.
Wojdylo A., Oszmianski J and Laskowski P. 2008. Polyphenolic compounds and antioxidant activity of new and old apple varieties. J. Agric. Food Chem. 56:6520-6530.
Wu J., Gao H., Zhao L., Liao X., Chen F., Wang Z. and Hu X. 2007. Chemical compositional characterization of some apple cultivars. Food Chem. 103:88-93.