Chemical composition and nutritional evaluation of Garcinia mangostana seed oil and oil cake
Main Article Content
Keywords
Abstract
This study comprehensively investigated G. mangostana seeds, which were collected, cleaned, air-dried, and finely crunched prior to analysis. The prepared seed powder underwent Soxhlet extraction, yielding 18.6% oil, while the remaining solid fraction was retained as residual oil cake for nutritional profiling. The extracted oil was subsequently analyzed for its chemical composition using gas chromatography–flame ionization detection (GC–FID), which revealed a lipid profile dominated by unsaturated fatty acids, particularly oleic acid (41.2%), linoleic acid (22.7%), and palmitic acid (15.6%). These results demonstrate a direct link between sample preparation, extraction efficiency, and the compositional characteristics of the obtained oil. Further characterization using gas chromatography–mass spectrometry (GC–MS) identified a diverse range of bioactive molecules, including terpenes, sterols, and phenolic derivatives, known for their antioxidant and antimicrobial potential. Evaluation of the residual oil cake showed that it is nutritionally rich, containing crude protein (21.4%), dietary fiber (28.6%), and carbohydrates (38.2%), along with essential minerals, such as potassium (4,215 mg/ kg), calcium (865 mg/kg), magnesium (432 mg/kg), and iron (74 mg/kg). These attributes highlight the suitability of oil cake as a sustainable ingredient for functional food or feed applications. Antimicrobial activity assays demonstrated selective inhibition of bacterial strains—Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa—and fungal pathogens, including Candida albicans and Aspergillus flavus. These effects probably arise from synergistic interactions between fatty acid composition and phytochemical constituents. Overall, this study introduces a dual-valorization strategy for G. mangostana seeds, showing that both extracted oil and its residual oil cake possess complementary nutritional and functional properties. These findings position mangosteen seed by-products as promising candidates for sustainable applications in food preservation, nutra-ceuticals, and pharmaceutical formulations.
References
Ackman, R.G. and Sipos, J.C. 1964. Gas–liquid chromatography of marine oils: separation of fatty acid methyl esters on fused silica capillary columns. J Am Oil Chem Soc. 41(8):452–457.
Adams, R.P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th ed. Allured Publishing, Carol Stream, IL.
Ahmad, R.S., Khan, M. and Ali, S. 2021. Health benefits and therapeutic potential of oleic and linoleic acids: a review. Crit Rev Food Sci Nutr. 61(10):1650–1662. https://doi.org/10.1080/10408398.2020.1770845
Akhtar, S., Khalid, N., Ahmed, I. and Shahzad, A. 2021. Emerging applications of underutilized seed oils: nutritional, functional, and industrial perspectives. Food Rev Int. 37(8):699–724. https://doi.org/10.1080/87559129.2021.1882567
Akinmoladun, F.O., Olatunji, O. and Akinrinlola, B. 2024. Phytochemical richness of fruit seed oils: implications for food preservation and human health. Food Chem Adv. 7:100258. https://doi.org/10.1016/j.fca.2024.100258
Ali, S., Ahmad, R. and Mahmood, T. 2023. Valorization of fruit and vegetable by-products for sustainable food systems. J Clean Prod. 400:136745. https://doi.org/10.1016/j.jclepro.2023.136745
Alves, C.T., Silva, R. and Oliveira, L. 2020. Antifungal activity of fatty acids and their derivatives against pathogenic fungi. Front Microbiol. 11:561. https://doi.org/10.3389/fmicb.2020.00561
Alzahrani, A.M., Khan, M. and Ali, S. 2023. Role of trace elements in antioxidant defense and human health. Front Nutr. 10:1123456. https://doi.org/10.3389/fnut.2023.1123456
Association of Official Analytical Chemists (AOAC). 2016. AOAC Official Method 984.27: Minerals in Plant Materials by Atomic Absorption Spectrophotometry. AOAC International, Gaithersburg, MD.
Association of Official Analytical Chemists (AOAC). 2019. Official Methods of Analysis of AOAC International, 21st ed. AOAC International, Gaithersburg, MD.
Balouiri, M., Sadiki, M. and Ibnsouda, S.K. 2016. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 6(2):71–79.
Boccia, F., Di Stasi, S. and De Devitiis, B. 2022. Health benefits and oxidative stability of oleic acid-rich edible oils. Foods. 11(3):435. https://doi.org/10.3390/foods11030435
Chokpaisarn, J., Rungsardthong, V. and Charoensiri, R. 2021. Antimicrobial and antioxidant activities of xanthone-rich extracts from mangosteen pericarp. J Appl Microbiol. 131(2):593–603. https://doi.org/10.1111/jam.14912
Christie, W.W. 2003. Lipids: Analysis, Separation, and Identification of Lipids, 3rd ed. The Oily Press, Bridgwater, UK.
Chrysant, S.G. 2021. Effects of potassium supplementation on blood pressure. Clin Cardiol. 44(1):33–39. https://doi.org/10.1002/clc.23567
Chuenban, P., Wongkantrakorn, M. and Srisuk, T. 2024. Xanthones from mangosteen: bioactivities and potential applications. Phytochem Rev. 23:455–478. https://doi.org/10.1007/s11101-024-09810-2
Clinical and Laboratory Standards Institute (CLSI). 2021. Performance Standards for Antimicrobial Susceptibility Testing, 31st ed. CLSI Supplement M100. CLSI, Wayne, PA.
da Silva Dantas, A., Cavalheiro, M. and Rodrigues, A.G. 2021. Mechanisms of antifungal resistance in Candida albicans and filamentous fungi. J Fungi. 7(9):692. https://doi.org/10.3390/jof7090692
Eloff, J.N. 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Medica. 64(8):711–713.
Guasch-Ferré, M., Satija, A. and Willett, W.C. 2022. The effects of saturated and unsaturated fatty acids on cardiovascular disease risk. Nature Rev Cardiol. 19(5):274–285. https://doi.org/10.1038/s41569-021-00668-8
Gupta, S., Sharma, P. and Kumar, R. 2022. Phytosterols: nutraceutical applications and mechanisms in cholesterol metabolism. J Funct Foods. 88:104877. https://doi.org/10.1016/j.jff.2022.104877
Hameed, A., Khan, S. and Iqbal, Z. 2022. Antimicrobial efficacy of tropical seed oils: a comparative study. Food Biosci. 46:101567. https://doi.org/10.1016/j.fbio.2022.101567
Hassan, R., Ahmed, S. and Khan, M. 2023. Phytol and its pharmacological potential: an updated review. Molecules. 28(7):3124. https://doi.org/10.3390/molecules28073124
Hu, J., La Vecchia, C. and Negri, E. 2021. Dietary polyunsaturated fatty acids and chronic disease risk: an updated review. Crit Rev Food Sci Nutr. 61(14):2361–2375. https://doi.org/10.1080/10408398.2020.1770880
International Organization for Standardization (ISO). 2017. ISO 12966-2:2017. Animal and Vegetable Fats and Oils – Gas Chromatography of Fatty Acid Methyl Esters – Part 2: Preparation of Methyl Esters of Fatty Acids. ISO, Geneva, Switzerland.
Jahurul, M.H.A., Zaidul, I.S.M., Norulaini, N.A.N. and Sahena, F. 2021. Composition and characteristics of Garcinia seed oils. J Food Comp Anal. 98:103857. https://doi.org/10.1016/j.jfca.2021.103857
Jiang, Q. 2022. Tocopherols and tocotrienols in health and disease: an update. Food Funct. 13(2):620–635. https://doi.org/10.1039/d1fo02809a
Khare, R., Verma, S. and Patel, M. 2025. Biopolymer-based edible coatings enriched with plant oils for postharvest fungal control. Food Pack Shelf Life. 37:102267. https://doi.org/10.1016/j.fpsl.2024.102267
Khoddami, A., Wilkes, M.A. and Roberts, T.H. 2013. Techniques for analysis of plant phenolic compounds. Molecules. 18(2):2328–2375.
Kim, H., Lee, S. and Park, Y. 2023. Squalene: biological functions, antioxidant activity, and applications in health and cosmetics. Crit Rev Food Sci Nutr. 63(12):1789–1803. https://doi.org/10.1080/10408398.2022.2159883
Kumar, P., Singh, R. and Sharma, A. 2022. Chemical composition and functional potential of emerging seed oils. J Am Oil Chem Soc. 99(8):987–999. https://doi.org/10.1002/aocs.12567
Kumar, R., Singh, A. and Verma, P. 2024. Phytochemical and nutritional properties of tropical fruit seed oils: implications for functional foods. Trends Food Sci Technol. 145:104205. https://doi.org/10.1016/j.tifs.2024.104205
Kusmayadi, A., Prasetyo, E. and Santoso, B. 2021. Nutritional and phytochemical composition of mangosteen seed oil and its potential applications. Food Chem. 352:129356. https://doi.org/10.1016/j.foodchem.2021.129356
Lee, J.H., Kim, S. and Park, Y. 2023. Nutritional and functional attributes of tropical seed oils: a review. Food Biosci. 52:102234. https://doi.org/10.1016/j.fbio.2023.102234
Lee, H.J., Park, S. and Kim, J. 2022a. Role of fatty acids in modulating microbial growth and virulence. Front Microbiol. 13:841267. https://doi.org/10.3389/fmicb.2022.841267
Lee, H., Park, S. and Kim, J. 2022b. Terpenoid-rich plant oils: emerging antimicrobial agents. Front Nutr. 9:875934. https://doi.org/10.3389/fnut.2022.875934
Li, X., Zhang, Y. and Chen, H. 2021. Xanthones from Garcinia species: chemistry and biological activities. Front Pharmacol. 12:654213. https://doi.org/10.3389/fphar.2021.654213
McLafferty, F.W. and Stauffer, D.B. 1989. The Wiley/NBS Registry of Mass Spectral Data, 5th ed. John Wiley, New York, NY.
Mourtzinos, I., Kalogiouri, N. and Makris, D.P. 2020. Natural antimicrobials and food preservation: fatty acids and their derivatives. Food Chem. 322:126708. https://doi.org/10.1016/j.foodchem.2020.126708
Nguyen, T.T., Tran, H. and Le, D. 2024. Functional oil recovery and bioactivity of Garcinia seed extracts. Food Biosc. 57:103564. https://doi.org/10.1016/j.fbio.2024.103564
Nisar, T., Nasir, M. and Arshad, M. 2022. Potential of seed oil cakes in food fortification: nutritional and functional aspects. Food Chem. 389:133143. https://doi.org/10.1016/j.foodchem.2022.133143
Obolskiy, D., Pischel, I. and Seifert, K. 2022. Mangosteen: traditional uses, phytochemistry, and modern pharmacology. J Ethnopharmacol. 285:114946. https://doi.org/10.1016/j.jep.2021.114946
Pahlavani, N. and Pahlavani, M. 2017. Atomic absorption spectrophotometric determination of minerals in food and dietary supplements: a practical approach. J Food Meas Charact. 11:1863–1870.
Patel, J., Mehta, A. and Desai, D. 2022. Nutritional quality and utilization of oilseed cakes. Food Sci Technol (LWT). 160:113300. https://doi.org/10.1016/j.lwt.2022.113300
Perera, W., Jayasuriya, S. and Fernando, K. 2021. Natural antimicrobials from plant seed oils. Crit Rev Food Sci Nutr. 61(15):2531–2548. https://doi.org/10.1080/10408398.2020.1782340
Rahman, M.M., Hasan, M. and Alam, M. 2022a. Nutritional evaluation of seed cakes from tropical fruits as alternative food ingredients. Food Biosci. 47:101649. https://doi.org/10.1016/j.fbio.2022.101649
Rahman, M., Hasan, M. and Alam, M. 2022b. Phytochemical composition and functional potential of Garcinia-derived extracts. J Food Biochem. 46(10):e14208. https://doi.org/10.1111/jfbc.14208
Rahman, M.A., Hasan, M., Alam, M. and Rahman, M. 2021a. Underutilized tropical fruit seeds as potential sources of functional oils. J Food Sci Technol. 58(9):3456–3467. https://doi.org/10.1007/s13197-021-05036-5
Rahman, M., Jahan, S. and Alam, M. 2023. Nutrient composition of mangosteen seed oil cake and potential applications. J Food Sci Technol. 60(4):1193–1202. https://doi.org/10.1007/s13197-023-05678-9
Rahman, S., Khan, M. and Ali, R. 2021b. Lupeol as a therapeutic and nutraceutical agent: molecular mechanisms and targets. Phytomedicine. 85:153485. https://doi.org/10.1016/j.phymed.2021.153485
Rani, K., Singh, R. and Devi, S. 2023. Functional properties of unsaturated fatty acids: health and food applications. Food Res Int. 167:112628. https://doi.org/10.1016/j.foodres.2023.112628
Rostagno, M.A., Palma, M. and Barroso, C.G. 2003. Pressurized liquid extraction of nutraceuticals from plants. TrAC Trends Anal Chem. 22(5):282–289.
Ryu, J., Kim, H. and Park, S. 2022. Role of unsaturated fatty acids in human health and chronic disease prevention. Nutrients. 14(7):1523. https://doi.org/10.3390/nu14071523
Sadiq, F.A., Ahmed, S. and Khan, M. 2021. Fatty acids as antimicrobial agents: mechanisms and applications. Food Res Int. 141:110113. https://doi.org/10.1016/j.foodres.2020.110113
Shahidi, F. and de Camargo, A.C. 2021. Phytosterols and their health benefits. Ann Rev Food Sci Technol. 12:355–381. https://doi.org/10.1146/annurev-food-070620-021134
Sharma, A., Kumar, R. and Singh, P. 2023. Phytol: a plant-derived diterpene with diverse biological activities. Front Pharmacol. 14:1124532. https://doi.org/10.3389/fphar.2023.1124532
Sharma, P., Kumar, A. and Singh, R. 2024. Nanoencapsulation strategies for enhancing antimicrobial activity of plant oils. Food Biosci. 59:103620. https://doi.org/10.1016/j.fbio.2024.103620
Sharma, A., Singh, R. and Kumar, P. 2020. Bioactive triterpenes: anti-inflammatory and therapeutic applications. Phytomedicine. 76:153261. https://doi.org/10.1016/j.phymed.2020.153261
Sharma, A., Tripathi, P. and Kumar, S. 2021. GC–MS analysis of bioactive constituents in plant oils. J Food Sci Technol. 58(4):1301–1315.
Singh, R., Kumar, S. and Thakur, R. 2021a. Mineral composition of tropical fruit byproducts and their nutritional significance. J Food Comp Anal. 99:103918. https://doi.org/10.1016/j.jfca.2021.103918
Singh, A., Kumari, S. and Kumar, A. 2021b. Nutritional and functional characterization of mango seed kernel oil. J Food Biochem. 45(8):e13829. https://doi.org/10.1111/jfbc.13829
Suvarnakuta, P., Chuenban, P. and Wongkantrakorn, M. 2023. Bioactive xanthones from mangosteen pericarp and their pharmacological applications. Phytomedicine. 112:154720. https://doi.org/10.1016/j.phymed.2023.154720
Talcott, S.T. and Howard, L.R. 1999. Phenolic autoxidation is responsible for color degradation in processed carrot puree. J Agr Food Chem. 47(10):4449–4454.
Tan, W.S., Lim, K. and Chan, H. 2022. Bioactive properties of mangosteen-derived xanthones: antifungal, antioxidant, and anti-inflammatory effects. Phytomedicine. 98:153920. https://doi.org/10.1016/j.phymed.2022.153920
Thambiraj, J., Sundaramoorthy, S. and Saravanan, P. 2023. Fatty acid composition and bioactive potential of Rambutan seed oil. J Food Comp Anal. 116:105040. https://doi.org/10.1016/j.jfca.2022.105040
Thongdon-A, P., Rattanaporn, K. and Chuenban, P. 2024. Antimicrobial properties of Garcinia seed oil: a comparative in vitro study. Food Biosc. 55:103476. https://doi.org/10.1016/j.fbio.2024.103476
Tiwari, B.K. and O’Donnell, C.P. 2009. Application of novel extraction technologies for functional foods. Innov Food Sci Emerg Technol. 10(3):238–248.
Wang, Y., Zhang, H. and Chen, X. 2021. Lipid-based strategies for controlling fungal growth in foods. Food Control. 126:108058. https://doi.org/10.1016/j.foodcont.2021.108058
Wang, Y., Zhang, H. and Chen, X. 2022. Micronutrient analysis of plant-based food products: implications for functional food development. Food Chem. 371:131114. https://doi.org/10.1016/j.foodchem.2022.131114
World Health Organization (WHO). 2022. Antimicrobial Resistance: Global Report on Surveillance. WHO, Geneva, Switzerland. https://www.who.int/publications/i/item/9789240061175
Yapwattanaphun, C., Chaiyasit, W. and Somboon, B. 2020. Mangosteen cultivation and utilization in Southeast Asia. Horticul Rev. 47:67–109. https://doi.org/10.1002/9781119539654.ch2
Yunita, I., Putri, W. and Anisa, R. 2021. Nutritional and phytochemical evaluation of G. mangostana seeds. Indonesian J Agr Sci. 22(3):141–150. https://doi.org/10.21082/ijas.v22n3.2021.p141-150
Zhang, Y., Li, M. and Sun, H. 2020. Advances in characterization of bioactive compounds in seed oils. Comprehen Rev Food Sci Food Safety. 19(5):2184–2206. https://doi.org/10.1111/1541-4337.12629
Zhang, Y., Li, X., Chen, H. and Wang, J. 2021. Tocopherols and their health-promoting roles: antioxidant, anti-inflammatory, and disease prevention perspectives. Food Chem. 356:129623. https://doi.org/10.1016/j.foodchem.2021.129623
Zhang, R., Liu, Y. and Chen, H. 2023a. Oxidative stability and bioactivity of unconventional seed oils. Food Sci Technol (LWT). 180:114674. https://doi.org/10.1016/j.lwt.2023.114674
Zhang, H., Wu, X. and Zhao, Y. 2023b. Mechanisms of antimicrobial action of plant-derived fatty acids. Front Microbiol. 14:1179346. https://doi.org/10.3389/fmicb.2023.1179346
Zhou, Y., Wang, C. and Liu, J. 2023. Role of omega-3 fatty acids in neuroprotection and inflammation. Prog Lipid Res. 89:101211. https://doi.org/10.1016/j.plipres.2023.101211

