Is coffee powder extract a possible functional ingredient useful in food and nutraceutical industries?

Main Article Content

Ancuta Nartea
Paolo Lucci
Monica Rosa Loizzo
Rosa Tundis
Mariarosaria Leporini
Luigia Gervasi
Benedetta Fanesi
Oscar Núñez
Natale Giuseppe Frega
Dennis Fiorini
Deborah Pacetti

Keywords

antioxidant activity, coffee, ethanolic extract, hypoglycemic effect, phenols, tocopherols

Abstract

The present study aimed to assess the phytochemical content and in vitro bioactivity of ethanolic extracts of Arabica (A) and/or Robusta (R) coffee powder having different geographical origins. For this purpose, total phenols (TPC) and flavonoids (TFC) content as well as α- and β-tocopherol were quantified. The antioxidant activity was assessed by using a multi-target approach in which the radical scavenging potential, the protection from lipid peroxidation, and the involvement of the iron-reducing mechanism were applied. The carbohydrate hydrolyzing enzymes’ (α-amylase and α-glucosidase) inhibitory activities were also assessed. Arabica coffee sample (C2-A) showed the highest TPC, TFC, and α-tocopherol content with values of 63.1 mg chlorogenic acid equivalents (CAE)/g dry powder, 16.2 mg of quercetin (QE) equivalents/g dry powder, and 5.6 mg/100 g dry powder, respectively. Relative Antioxidant Capacity Index (RACI), used to statistically integrate results from 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability power (FRAP), and protection of lipid peroxidation assays, evidenced that sample C4-R derived from Robusta from Guatemala showed the highest antioxidant potential with a value of –0.61. Arabica from Puerto Rico was the most active against α-amylase, whereas the blend Arabica/Robusta sample (C5-A60R40) showed the highest inhibitory activity against α-glucosidase with IC50 values of 120.2 and 134.6 mg/mL, respectively. The results show how the qualitative-quantitative composition of the extracts is strongly associated not only with the variety but also with the geographical origin of the samples.

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References

Acidri, R., Sawai, Y., Sugimoto, Y., Handa, T., Sasagawa, D., Masunaga, T., et al. 2020. Phytochemical profile and antioxidant capacity of coffee plant organs compared to green and roasted coffee beans. Antioxidants 9: 93–111. 10.3390/antiox9020093

Alves, R.C., Casal, S., Alves, M.R. and Oliveira, M.B., 2009. Discrimination between Arabica and Robusta coffee species on the basis of their tocopherol profiles. Food Chemistry 114: 295–299. 10.1016/j.foodchem.2008.08.093

Balzano, M., Loizzo, M.R., Tundis, R., Lucci, P., Nunez, O., Fiorini, D., et al. 2020. Spent Espresso coffee grounds as a source of anti-proliferative and antioxidant compounds. Innovative Food Science and Emerging Technologies 59: 102254. 10.1016/j.ifset.2019.102254

Bobková, A., Hudáček, M., Jakabová, S., Belej, Ľ., Capcarová, M., Čurlej, J., et al. 2020. The effect of roasting on the total polyphenols and antioxidant activity of coffee. Journal of Environmental Science and Health 55: 495–500. 10.1080/03601234.2020.1724660

Carrier, A., 2017. Metabolic syndrome and oxidative stress: a complex relationship. Antioxidants Redox Signaling 26: 429–431. 10.1089/ars.2016.6929

Directive 2009/32/EC of the European Parliament and of the Council of 23 April 2009 on the approximation of the laws of the Member States on extraction solvents used in the production of foodstuffs and food ingredients. Official Journal of the European Union 141/3-11. Access January 2019.

Dórea, J.G. and da Costa, T.H., 2005. Is coffee a functional food? British Journal of Nutrition 93: 773–782. 10.1079/bjn20051370

Farah, A. and de Paula Lima, J., 2019. Consumption of chlorogenic acids through coffee and health implications. Beverages 5: 11–40. 10.3390/beverages5010011

Giardinieri, A., Schicchi, R., Geraci, A., Rosselli, S., Maggi, F., Fiorini, D., et al. 2019. Fixed oil from seeds of narrow-leaved ash (F. angustifolia subsp. angustifolia): chemical profile, antioxidant and antiproliferative activities. Food Research International 119: 369–377. 10.1016/j.foodres.2019.02.013

Global Nutraceuticals Market Size, Analysis, Trends, Industry Statistics (2019–2024). Available at: https://www.mordorintelligence.com/industry-reports/global-nutraceuticals-market-industry

González, A.G., Pablos, F., Martín, M.J., León-Camacho, M. and Valdenebro, M.S., 2001. HPLC analysis of tocopherols and triglycerides in coffee and their use as authentication parameters. Food Chemistry 73: 93–101. 10.1016/S0308-8146(00)00282-X

Górnaś, P., Siger, A., Pugajeva, I., Czubinski, J., Waśkiewicz, A. and Polewski, K., 2014. New insights regarding tocopherols in Arabica and Robusta species coffee beans: RP-UPLC-ESI/MSn and NP-HPLC/FLD study. Journal of Food Composition and Analysis 36: 117–123. 10.1016/j.jfca.2014.08.005

Herawati, D., Giriwono, P., Dewi, F., Kashiwagi, T. and Andarwulan, N., 2019. Antioxidant, anti-α-glucosidase and anti-glycation activities of coffee brew from Robusta coffee beans roasted at different levels. International Food Research Journal 26: 1305–1313.

International Coffee Organization, 2019. Growing for prosperity. Economic viability as the catalyst for a sustainable coffee sector, coffee development report 2019. Available at: https://www.internationalcoffeecouncil.org/media/coffeeDevelopmentReport.pdf Coffee market report 2021. Accessed 11 May 2021.

International Diabetes Federation, 2019. IDF diabetes atlas. 9th ed. pp. 1–176, Brussels, Belgium.

Jeon, J.S., Kim, H.T., Jeong, I.H., Hong, S.R., Oh, M.S., Yoon, M.H., et al. 2019. Contents of chlorogenic acids and caffeine in various coffee-related products. Journal of Advanced Research 17: 85–94. 10.1016/j.jare.2019.01.002

Jeszka-Skowron, M., Sentkowska, A., Pyrzyńska, K. and De Peña, M.P., 2016. Chlorogenic acids, caffeine content and antioxidant properties of green coffee extracts: influence of green coffee bean preparation. European Food Research and Technology 242: 1403–1409. 10.1007/s00217-016-2643-y

Kròl, K., Gantner, M., Tatarak, A. and Hallmann, E., 2020. The content of polyphenols in coffee beans as roasting, origin and storage effect. European Food Research and Technology 246: 33–39. 10.1007/s00217-019-03388-9

Loizzo, M.R., Bonesi, M., Nabavi, S.M., Sobarzo-Sánchez, E., Rastrelli, L. and Tundis, R., 2017. Hypoglycaemic effects of plants food constituents via inhibition of carbohydrate-hydrolysing enzymes: from chemistry to future applications. In: Natural Products Targeting Clinically Relevant Enzymes. Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, pp. 135–161. 10.1002/9783527805921.ch6

Loizzo, M.R., Sicari, V., Tundis, R., Leporini, M., Falco, T. and Calabrò, V., 2019. The influence of ultrafiltration of Citrus Limon L. Burm. cv Femminello comune juice on its chemical composition and antioxidant and hypoglycemic properties. Antioxidants 8: 23. 10.3390/antiox8010023

Naveed, M., Hejazi, V., Abbas, M., Kamboh, A.A., Khan, G.J., Shumzaid, M., et al. 2018. Chlorogenic acid (CGA): a pharmacological review and call for further research. Biomedicine & Pharmacotherapy 97: 67–74. 10.1016/j.biopha.2017.10.064

Nyambe-Silavwe, H. and Williamson, G., 2018. Chlorogenic and phenolic acids are only very weak inhibitors of human salivary α-amylase and rat intestinal maltase activities. Food Research International 113: 452–455. 10.1016/j.foodres.2018.07.038

Oboh, G., Agunloye, O.M., Adefegha, S.A., Akinyemi, A.J. and Ademiluyi, A.O., 2015. Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study. Journal of Basic and Clinical Physiology and Pharmacology 26: 165–170. 10.1515/jbcpp-2013-0141

Ong, K.W., Hsu, A. and Tan, B.K.H., 2013. Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by AMPK Activation. Biochemical Pharmacology 85: 1341–1351. 10.1016/j.bcp.2013.02.008

Osama, H., Abdelrahman, M.A., Madney, Y.M., Harb, H.S., Saeed, H. and Abdelrahim, M.E.A., 2021. Coffee and type 2 diabetes risk: is the association mediated by adiponectin, leptin, C-reactive protein or interleukin-6? A systematic review and meta-analysis. International Journal of Clinical Practice 75: e13983. 10.1111/ijcp.13983

Socaci, S., Fărcaş, A., Diaconeasa, Z., Vodnar, D.C., Rusu, B. and Tofana, M., 2018. Influence of the extraction solvent on phenolic content, antioxidant, antimicrobial and antimutagenic activities of brewers’ spent grain. Journal of Cereal Science 80: 180–187. 10.1016/j.jcs.2018.03.006

Sulaiman, S.F., Moon, J.K. and Shibamoto, T., 2011. Investigation of optimum roasting conditions to obtain possible health benefit supplement, antioxidants from coffee beans. Journal of Dietary Supplements 8: 293–310. 10.3109/19390211.2011.593618

Tirzitis, G. and Bartosz, G., 2010. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochimica Polonica 57: 139–142. 10.18388/abp.2010_2386