CHARACTERIZATION OF VOLATILE COMPOUNDS IN FIVE BLUEBERRY VARIETIES USING PURGE AND TRAP COUPLED TO GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Main Article Content

Z. XINYU
W. YI
H. XUE
Y. ZIXI
Y. WEIQIONG
L. ZHAOLIN

Keywords

aroma active compounds, blueberry, GC-MS, P&T, volatile compounds

Abstract

The volatile composition of five blueberry varieties from two different regions was analysed by dynamic headspace (purge and trap, P&T)  coupled to gas chromatography-mass spectrometry (GC-MS). Under the optimized conditions, the P&T method was successfully validated, showing good linearity, high accuracy, good reproducibility and a low limit of detection. A total of 80 volatiles were identified, including 19 esters, 30 alcohols, 18 aldehydes, 7 ketones and 6 other compounds. Furthermore, a spider web diagram was constructed to compare the flavour profiles of these blueberries, and the obtained results demonstrated that blueberries from different locations have different flavour profiles.

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References

Aznar M. and Arroyo T. 2007. Analysis of wine volatile profile by purge-and-trap–gas chromatography–mass spectrometry. J. Chromatogr. A. 1165:151-157.

Beaulieu J.C., Stein-Chisholm R.E. and Boykin D.L. 2014. Qualitative analysis of volatiles in rabbiteye blueberry cultivars at various maturities using rapid solid-phase microextraction. J. Amer. Soc. Hort. Sci. 139:167-177.

Campillo N., Viñas P., López-Garc??a I., Aguinaga N. and Hernández-Córdoba M. 2004. Purge-and-trap capillary gas chromatography with atomic emission detection for volatile halogenated organic compounds determination in waters and beverages. J. Chromatogr. A. 1035:1-8.

Deng X., Liang G., Chen J., Qi M. and XieP. 2011. Simultaneous determination of eight common odors in natural water body using automatic purge and trap coupled to gas chromatography with mass spectrometry. J. Chromatogr. A. 1218:3791-3798.

Du X. and Rouseff R. 2014. Aroma active volatiles in four Southern highbush blueberry varieties determined by gas chromatography–olfactometry (GC-O) and gas chromatography-mass spectrometry (GC-MS). J Agric. Food Chem. 62:4537-4543

Gu S.Q., Tao N.P., Wu U. and Zhang J.J. 2012. A new method based on ROAV value to identify the characteristic key volatile compounds of crab flavor. Sci. Technol. Food Indus. 33:410-16.

Hakala M.A., Lapveteläinen A.T. and Kallio H.P. 2002. Volatile compounds of selected strawberry varieties analyzed by purge-and-trap headspace GC-MS. J. Agri. Food Chem. 50:1133-1142

Hall I.V., Forsyth F.R. and Lightfoot H.J. 1970. Volatiles from developing fruit of Vaccinium angustifolium. Can. Inst. Food Sci. Technol. 3:1-3

He Q. and Wu L.R. 2010. Biological functions of nutrients in blueberry fruits. Nor Horticul. 24:222-224.

Horvat R.J. and Senter S.D. 1985. Comparison of the volatile constituents from rabbiteye blueberries (Vaccinium ashei) during ripening. J. Food Sci. 50:429-431.

Larreta J., Bilbao U., Vallejo A., Usobiaga A., Arana G. and Zuloaga O. 2008. Multisimplex optimisation of the purge-and-trap preconcentration of volatile fatty acids, phenols and Indoles in cow slurries. Chromatogr. A. 67:93-99

Lefngwell, and Associates, www.lefngwell.com/odorthre.htm

Lv Z.L. and Lin X. 2012. Antioxidant activity of bamboo-leaf extracts from the species Dendrocalamopsisoldhami. Sci. Res. Essays. 44:3789-3796.

Murat, C., Gourrat, K., Jerosch, H., and Cayot, N. 2012. Analytical comparison and sensory representativity of SAFE, SPME, and purge and trap extracts of volatile compounds from pea flour. Food Chem. 135:913-920.

Parliament T.H. and Kolor M.G. 1975. Identification of the major volatile components of blueberry. J. Food Sci. 40:762-763.

Pino J.A. and Queris O. 2010. Analysis of volatile compounds of pineapple wine using solid-phase microextraction techniques. Food Chem. 122:1241-1246.

Silva S., Costa E.M., Calhau C., Morais R.M. and Pintado M.M.E. 2017. Production of a food grade blueberry extract rich in anthocyanins: selection of solvents, extraction conditions and purification method, J. Food Meas. Charact. 11:1248-1253.

Soria A.C., Martínez-Castro I. and Sanz J. 2009. Study of the precision in the purge-and-trap–gas chromatography-mass spectrometry analysis of volatile compounds in honey. J. Chromatogr. A. 1216:3300-3304.

Sun S.Y., Che C.Y., Sun T.F., Lv Z.Z., He S.X., Gu H.N., Shen W.J., Chi D.C. and Gao Y. 2013. Evaluation of sequential inoculation of Saccharomyces cerevisiae and Oenococcus oeni strains on the chemical and aromatic profiles of cherry wines. Food Chem. 138:2233-2241.

Wang Y., Yang C., Li S., Yang L., Wang Y., Zhao J. and Jiang Q. 2009. Volatile characteristics of 50 peaches and nectarines evaluated by HP-SPME with GC-MS. Food Chem. 116:356-364.

Yi, Z., Feng, T., Zhuang, H., Ye, R., Li, M., and Liu, T. 2016. Comparison of different extraction methods in the analysis of volatile compounds in pomegranate juice. Food Anal Method. 9:2364-2373.

Zhuang K., Wu N., Wang X., Wu X., Wang S., Long X. and Wei X. 2008. “ROAV” method: a new method for determining key odor compounds of Rugao ham. Food Sci. 29:370-374.