INFLUENCE OF EXOPOLYSACCHARIDE ON THE GROWTH OF LACTIC ACID BACTERIA

Main Article Content

H. TSUDA
S. OKUDA
T. HARAGUCHI
K. KODAMA

Keywords

exopolysaccharide, growth enhancement, Lactobacillus, yeast extract

Abstract

The highest production of exopolysaccharides (EPSs) by Lactobacillus buchneri GM3701 and Lb. plantarum RB-3 was 216 and 79.0 mg/L, when incubated in 10% glucose media at 25°C for 6 d and 5% glucose media at 25°C for 4 d, respectively. The EPSs consisted of mainly glucose. Bacterial growth in the media supplemented with the EPSs was investigated using various bacteria, including Lactobacillus, Staphylococcus and Escherichia strains. The EPS enhanced the growth of Lb. farciminis HM2001. This result suggests that the growth of some lactic acid bacteria can be enhanced by the supplementation with an EPS produced by Lactobacillus strains.

Abstract 386 | pdf Downloads 327

References

Adachi S. 1965. Thin-layer chromatography of carbohydrates in the presence of bisulfite. J. Chromatogr. 17:295-299.

Bradford M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.

van den Broek L.A.M., Hinz S.W.A., Beldman G., Vincken J.-P. and Voragen A.G.J. 2008. Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics. Mol. nutr. Food Res. 52:146-163.

Caggianiello G., Kleerebezem M. and Spano G. 2016. Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms. Appl. Microbiol. Biotechnol. 100:3877-3886.

Cerning J. 1990. Exocellular polysaccharides produced by lactic acid bacteria. FEMS Microbiol. Rev. 87:113-130.

Cerning J. 1995. Production of exopolysaccharides by lactic acid bacteria and dairy propionibacteria. Lait 7:463-472.

Chapot-Chartier M.P. and Kulakauskas S. 2014. Cell wall structure and function in lactic acid bacteria. Microb. Cell Fact. 13:S9.

Donot F., Fontana A., Baccou J.C. and Schorr-Galindo S. 2012. Microbial exopolysaccharides: Main examples of synthesis, excretion, genetics and extraction. Carbohydr. Polym. 87:951-962.

Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A. and Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356.

Gibson G.R. and Roberfroid M.B. 1995. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J. Nutr. 125:1401-1412.

Hashiguchi K., Nagata Y., Yoshida M., Murohushi Y. and Kitazawa H. 2011. Chemical and immunological characteerization of extracellular polysaccharides produced by Lactobacillus plantarum No.14. Jpn. J. Lactic Acid Bact. 22:100-105.

Hongpattarakere T., Cherntong N., Wichienchot S., Kolida S. and Rastall R.A. 2012. In vitro prebiotic evaluation of exopolysaccharides produced by marine isolated lactic acid bacteria. Carbohy. Poly. 87:846-852.

Huebner J., Wehling R.L. and Hutkins R.W. 2007. Functional activity of commercial prebiotics. Int. Dairy J. 17:770-775. Korakli M., Ganzle M.G. and Vogel R.F. 2002. Metabolism by bifidobacteria and lactic acid bacteria of polysaccharides from wheat and rye, and exopolysaccharides produced by Lactobacillus sanfranciscensis. J. Appl. Microbiol. 92:958-965.

Lindsay P.H., Valerie M.M., Mark E., Yucheng G. and Andrew P.L. 2003. Structural characterisation of a perdeuteriomethylated exopolysaccharide by NMR spectroscopy: characterisation of the novel exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus EU23. Carbohydr. Res.338:61-67.

Liu Z., Zhang Z., Qiu L., Zhang F., Xu X., Wei H. and Tao X. 2017. Characterization and bioactivities of the exopolysaccharide from a probiotic strain of Lactobacillus plantarum WLPL04. J. Dairy Sci. 100:1-11.

Macedo M.G., Lacroix C., Gardner N.J. and Champagne C.P. 2002. Effect of medium supplementation on exopolysaccharide production by Lacctobacillus rhamnosus RW-9595M in whey permeate. Int. Dairy J. 12:419-426.

Maeda H., Zhu X., Suzuki S., Suzuki K. and Kitamura S. 2004. Structural characterization and biological activities of an exopolysaccharide kefiran produced by Lactobacillus kefiranofaciens WT-2BT. J. Agric. Food Chem. 52:5533-5538.

Pham P.L., Dupoint I., Roy D., Lapointe G. and Cerning J. 2000. Production of exopolysaccharide by Lactobacillus rhamnosus R and analysis of its enzymatic degradation during prolonged fermentation. Appl. Environ. Microbiol. 66:2302-2310.

Ruijssenaars H., Stingele F. and Hartmans S. 2000. Biodegradability of food-associated extracellular polysaccharides. Curr. Microbiol. 40:194-199.

Russo P., Lopez P., Capozzi V., de Palencia P.F., Duenas M.T., Spano G. and Fiocco D. 2012. Beta-glucans improve growth, viability and colonization of probiotic microorganisms. Int. J. Mol. Sci. 13:6026-6039.

Sanchez J.I., Martinez B., Guillen R., Jimenez-Diaz R. and Rodriguez A. 2006. Culture conditions detemine the balance between two different exopolysaccharides produced by Lactobacillus pentosusLPS26. Appl. Environ. Microbiol. 72:7495-7502.

Staaf M., Yang Z., Huttunen E. and Widmalm G. 2000. Structural elucidation of the viscous exopolysaccharide produced by Lactobacillus helveticus Lb161. Carbohydr. Res. 326:113-119.

Tsuda H. and Miyamoto T. 2010. Production of exopolysaccharide by Lactobacillus plantarum and the prebiotic activity of the exopolysaccharide. Food Sci. Technol. Res. 16:87-92.

Tsuda H., Matsumoto T. and Ishimi Y. 2012. Selection of lactic acid bacteria as starter cultures for fermented meat products. Food Sci. Technol. Res. 18:713-721.

Tsuda H. 2015. Identification of lactic acid bacteria from raw milk of Wagyu cattle and their tolerance to simulated digestive juice. Milk Sci. 64:207-214.

de Vuyst L., de Vin F., Vaningelgem F. and Degeest B. 2001. Recent developments in the biosynthesis and applications of heteropolysaccharides from lactic acid bacteria. Int. Dairy J. 11:687-707.