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L. monocytogenes, biofilms, sanitizer, ultrasound, food industry


This work aimed at studying methods for Listeria monocytogenes attached cells removal from food industry equipment. In order to verify possible contamination points, a tracking was carried out in food products and equipment surfaces (brine injection needles) by swab. Brine injection needles were defined as a study due to their higher contamination potential. L. monocytogenes adhesion after 6 h contact with needles was observed. Minimum bactericidal concentration (MBC) of peracetic acid on L. monocytogenes attached cells was 0.06 and 0.24% with 6 and 24 h contact with bacteria, respectively. 75% power (700 W) and 14 min exposure ultrasound treatment showed a 3.25 Log CFU microbial load reduction in injection needles. The combination of ultrasound and peracetic acid showed an MBC reduction from 0.24 to 0.03%, hence, it may be recommended for meat industry application.

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AOAC (Association of Official Analytical Chemists) (2016) Official method, 2011.03 - Salmonella in a Variety of Food-VIDAS® Salmonella (SLM) Easy Salmonella Method, 20th ed.

Ban G.H. and Kang D.H. 2015. Effect of sanitizer combined with steam heating on the inactivation of foodborne pathogens in a biofilm on stainless steel. Food Microbiol. 55:47-54.

Banach J.L. Sampers I. Van Haute S. and Van Der Fels-Klerx H.J. 2015. Effect of disinfectants on preventing the cross-contamination of pathogens in fresh produce washing water. Int. J. Environ.Res. Public Health 12 (8):8658-8677.

Barbosa J. Cuppini M. Flach J. Steffens C. Cansian R.L. and Toniazzo G. 2016. Removal of Escherichia coli in boning knives with diferente sanitizers. LWT-Food Sci. Technol. 71:309-315.

Belessi C-E.A. Gounadaki A.S. Psomas A.N. and Skandamis P.N. 2011. Efficiency of different sanitation methods on Listeria monocytogenes biofilms formed under various environmental conditions. Int. J. Food Microbiol. 1:46-52.

Beltrame C.A. Martelo E.B. Mesquita R. Rottava I. Barbosa J. Steffens C. Toniazzo G. Valduga E. and Cansian R.L. 2016. Effectiveness of sanitizing agents in inactivating Escherichia coli (ATCC 25922) in food cutting board surfaces. Ital. J. Food Sci.28:148-154.

Betts G.D. Williams A. and Oakley R.M. 2014. Ultrasonic Standing Waves: Inactivation of Foodborne Microorganisms Using Power Ultrasound. Encyclopedia Food Microbiol. 2:659-664.

Branda S.S. Vik S. Friedman L. and Kolter R. 2005. Biofilms: the matrix revisited. Trends Microbiol. 13(1):20-6.

Brasil C.C.B. Barin J.S. Jacob-Lopes E. Menezes C.R. Zepka L.Q. Wagner R., Campagnol P.C.B. and Cichoski A.J. 2017. Singles step non-thermal cleaning/sanitation of knives used in meat industry with ultrasound. Food Res. Int. 91:133-139.

Brondum J. Egebo M. Agerskov C. and Busk H. 1998. Online pork carcass grading with the autoform ultrasound system. J. Anim. Sci. 76:1859-1868.

Butz P. and Tauscher B. 2002. Emerging technologies: chemical aspects. Food Res. Int. 35:279-284.

Carpentier B. and Cerf O. 2011. Review -Persistence of Listeria monocytogenes in food industry equipment and premises. Int. J. Food Microbiol. 145 (1):1-8.

Chavant P. Gaillard-Martinie B. Talon R. Hébraud M. and Bernardi T. 2007. New device for rapid evaluation of biofilm formation potential by bacteria. J. Microbiol. Method. 68 (3):605-612.

Erriu M. Blus C. Szmukler-Moncler S. Buogo S. Levi R. Barbato G. Manonnaripa D. Denotti G. Piras V. and Orrù G. 2014. Microbial biofilm modulation by ultrasound: Current concepts and controversies. Ultrason. Sonochem. 21:15-22.

Henriques A.R. Gama L.T. and Fraqueza M.J. 2017. Tracking Listeria monocytogenes contamination and virulence-associated characteristics in the ready-to-eat meat-based food products industry according to the hygiene level. Int. J. Food Microbiol. 242:101-106.

Hori K. and Matsumoto S. 2010. Bacterial adhesion: From mechanism to control. Biochem. Eng. J. 48:424-434.

ISO 11290-1. 1996. Microbiology of food and animal feeding stuffs -Horizontal method for the detection and enumeration of Listeria monocytogenes- Part 1: Detection method.

Jay J.M. 2005. Microbiologia de alimentos. 6. ed. Porto Alegre: Artmed.

José J.F.B. and Vanetti M.C.D. 2012. Effect of ultrasound and commercial sanitizers in removing natural contaminants and Salmonella enterica Typhimuriumon cherry tomatoes. Food Control 24:95-99.

Luo K. and Oh D-H. 2016. Inactivation kinetics of Listeria monocytogenes and Salmonella enterica serovar Typhimurium on fresh-cut bell pepper treated with slightly acidic electrolyzed water combined with ultrasound and mild heat. Food Microbiol. 53:165-171.

Malheiros J. and Simões M. 2017. Antimicrobial resistance of biofilms in medical devices. In: Biofilms and Implantable Medical Devices. Ying Deng and Wei Lv, Woodhead Publishing, 97-113.

Mcdonnell G. and Russell A.D. 1999. Antiseptics and disinfectants: activity, action, and resistance. Clin. Microbiol. Rev. 12:147-179.

Miks-Krajnik M. Feng L.X.J. Bang W.S. and Yuk H-G. 2017. Inactivation of Listeria monocytogenes and natural microbiota on raw salmon fillets using acidic electrolyzed water, ultraviolet light or/and ultrasounds. Food Control 74:54-60.

Myszka K. and Czaczyk K. 2001. Bacterial biofilms on food contact surfaces -a review. Pol. J. Food Nutr. Sci. 61 (3):173-180.

Pivasena P. Mohareb E. and Mckellar R.C. 2003. Inactivation of microbes using ultrasound: a review. Int. J. Food Microbiol. 87:207-216.

Poimenidou S.V. Chrysadakou M. Tzakoniati A. Bikouli V.C. Nychas G-J. and Skandamis P.N. 2016. Variability of Listeria monocytogenesstrains in biofilm formation on stainless and polystyrene materials and resistance to peracetic acid and quaternary ammonium compounds. Int. J. Food Microbiol. 237:164-171.

Rust R.E. and Knipe C.L. 2014. Processing Equipment/Brine Injectors. Encyclopedia of Meat Sciences, 2 ed, 123-125.

Scherba G. Weigel R.M. and O’Brien J.R. 1991. Quantitative assessment of the germicidal efficacy of ultrasonic energy. Appl. Environ. Microbial. 57 (7):2079-2084.

Srey S. Jahid I.K. and Do Ha S. 2013. Biofilm formation in food industries: A food safety concern. Food Control 31:572-585.

Turantas F. Kiliç G.B. and Kili B. 2015. Ultrasound in the meat industry: General applications and decontamination efficiency. Int. J. Food Microbiol. 198 (2):59-69.