ULTRASOUND USE FOR LISTERIA MONOCYTOGENES ATTACHED CELLS REMOVAL FROM INDUSTRIAL BRINE INJECTION NEEDLES

Main Article Content

D. HAMANN
K. F. TONKIEL
A. MATTHIENSEN
J. ZENI
E. VALDUGA
N. PAROUL
C. STEFFENS
G. TONIAZZO
R. CANSIAN

Keywords

L. monocytogenes, biofilms, sanitizer, ultrasound, food industry

Abstract

This work aimed at studying methods for Listeria monocytogenes attached cells removal from food industry equipment. In order to verify possible contamination points, a tracking was carried out in food products and equipment surfaces (brine injection needles) by swab. Brine injection needles were defined as a study due to their higher contamination potential. L. monocytogenes adhesion after 6 h contact with needles was observed. Minimum bactericidal concentration (MBC) of peracetic acid on L. monocytogenes attached cells was 0.06 and 0.24% with 6 and 24 h contact with bacteria, respectively. 75% power (700 W) and 14 min exposure ultrasound treatment showed a 3.25 Log CFU microbial load reduction in injection needles. The combination of ultrasound and peracetic acid showed an MBC reduction from 0.24 to 0.03%, hence, it may be recommended for meat industry application.

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