Main Article Content
fatty acid, fiber, myosin, quality, tenderness
The aims of this study were to examine the effects of myosin heavy chain (MHC) isoforms on Cinta Senese meat and sensory quality. The research was carried out on 65 pigs and muscle samples characteristics such as MHC isoform, meat quality, fatty acid composition, and sensory were evaluated. The results demonstrated that MHC slow isoform content was significantly correlated with pH24h (r=0.25, P<0.05) and drip loss (r=-0.31, P<0.005), whereas the content of MHC isoforms was only weakly correlated with fatty acids. Sensory evaluation was done by a trained panel test and the results shown that the MHC fast/slow ratio was correlated with the juiciness (r=-0.32, P<0.005), off-flavor (r=0.33, P<0.01), and tenderness attributes (r=-0.42 to -0.46). We therefore conclude that the content of MHC isoforms can be one of the most important factors for examination of overall quality of Cinta Senese pigs.
AOAC. 2000. Solvent extraction method for fat in meat 991.36. In: Official methods of analysis. 17th ed. Gaithersburgh, Maryland. Assoc. Off. Anal. Chem.
Behrends J.M., Goodson K.J., Koohmaraie M., Shackelford S.D., Wheeler T.L. and Morgan W.W. 2005. Beef customer satisfaction: USDA quality grade and marination effects on consumer evaluations of top round steaks. J. Anim. Sci. 83:662-670.
Bendall J.R. and Swatland H.J. 1988. A review of the relationships of pH with physical aspects of pork quality. Meat Sci. 24(2):85-126.
Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
Calkins C.R. and Hodgen J.M. 2007. A fresh look at meat flavour. Meat Sci. 77:63-80.
Choi Y.M. and Kim B.C. 2009. Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality. Livest Sci. 122:105-118.
Choi Y.M., Lee S.H., Choe J.H., Rhee M.S., Lee S.K., Joo S.T. and Kim B.C. 2010. Protein solubility is related to myosin isoforms, muscle fiber types, meat quality traits, and postmortem protein changes in porcine longissimus dorsi muscle. Livest Sci. 127:183-191.
Choi Y.M., Ryu Y.C. and Kim B.C. 2006. Effect of myosin heavy chain isoforms on muscle fiber characteristics and meat quality in porcine longissimus muscle. J. Muscle. Foods 17:413-427.
Commission Internationale De L’eclairage. 1978. Recommendations on uniform color spaces -color differences equations, psychrometic color terms. Supplement No. 2, Cie Publication No. 15, E1.3.1.
Dai F., Feng D., Cao Q., Ye H., Zhang C., Xia W. and Zuo J. 2009. Developmental differences in carcass, meat quality and muscle fibre characteristics between the landrace and a chinese native pig. S. Afr. J. Anim. Sci. 39(4).
Dall Aaslyng M., Bejerholm C., Ertbjerg P., Bertram H.C. and Andersen H.J. 2008. Cooking loss and juiciness of pork in relation to raw meat quality and cooking procedure. Anim. 2:1700-1706.
Dinh N.T.T. 2006. Meat quality: understanding of meat tenderness and influence of fat content on meat flavor. Sci. Tech. Develop. 9:65-70.
Eikelenboom G., Hoving-Bolink A.H. and Van Der Wal P.G. 1996. The eating quality of pork 2. The influence of intramuscular fat. Fleischt. 76:517-518.
European Commission, 2004a. Commission Regulation of the European Parliament and of the Council of 29 April 2004 on the hygiene of foodstuffs, 852/2004/EC. In: Official Journal, L 139/1, 30.4.2004.
European Commission, 2004b. Commission Regulation of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin, 853/2004/EC. In: Official Journal, L 139, 30.4.2004.
European Commission, 2004c.Commission Regulation of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organization of official controls on products of animal origin intended for human consumption, 854/2004/EC. In: Official Journal, L 139,30.4.2004.
European Commission, 2005e. Commission Regulation laying down transitional arrangements for the implementation of Regulations (EC) No. 853/2004, (EC) No. 854/2004, 2076/2005/EC. In: Official Journal, L 338/83, 22.12.2005.
Fernandez X., Levasseur P., Ecolan P. and Wittmann W. 1995. Effect of epinephrine administration on glycogen metabolism in red and white muscle of anaesthetized pigs (sus scrofa domesticus). J. Agric. Sci. 68:231-239.
Folch J., Lees M. and Slaone-Stanely G.H. 1957. A simple method for the isolation and purification of total lipid from animal tissue. J. Bio. Chem. 226:497-509.
Fortin A., Robertson W.M. and Tong A.K.W. 2005. The eating quality of canadian pork and its relationship with intramuscular fat. Meat Sci. 69:297-305.
Gil M., Oliver M.A., Gispert M., Diestre A., Sosnicki A.A., Lacoste A. and Carrion D. 2003. The relationship between pig genetics, myosin heavy chain I, biochemical traits and quality of m. Longissimus thoracis. Meat Sci. 65:1063-1070.
Granlund A., Jensen-Waern M. and Essén-Gustavsson B. 2011. The influence of the prkag3 mutation on glycogen, enzyme activities and fibre types in different skeletal muscles of exercise trained pigs. Acta Vet. Scan. 53:20.
Hintz C.S., Coyle E.F., Kaiser K. K., Chi M.M. and Lowry O.H. 1984. Comparison of muscle fiber typing by quantitative enzyme assays and bymyosin ATPase staining. J. Histochem. Cyotochem. 32:655-660.
Honikel K.O. 1987. How to measure the water-holding capacity of meat? Recommendation of standardized methods. In: P. V. Tarrant, G. Eikelenboom, and G. Monin, editors, Evaluation and control of meat quality in pigs. p. 129-142.
Huff-Lonergan E. and Lonergan S.M. 2005. Mechanisms of water-holding capacity of meat: the role of postmortem biochemical and structural changes. Meat Sci. 71:194-204.
Huff-Lonergan E., Baas T. J., Malek M., Dekkers J.C., Prusa K. and Rothschild M.F. 2002. Correlations among selected pork quality traits. J. Anim. Sci. 80:617-627.
Jeong D.W., Choi Y.M., Lee S.H., ChoeJ.H., Hong K.C., Park H.C. and Kim B.C. 2010. Correlations of trained panel sensory values of cooked pork with fatty acid composition, muscle fiber type, and pork quality characteristics in berkshire pigs. Meat Sci. 86:607-615.
Kang Y.K., Choi Y.M., Lee S.H., Choe J.H., Hong K.C. and Kim B.C. 2011. Effects of myosin heavy chain isoforms on meat quality, fatty acid composition, and sensory evaluation in berkshire pigs. Meat Sci. 89:384-389.
Karlsson A., Enfalt A.C., Essen-Gustavsson B., Lundstrom K., Rydhmer L. and Stern S. 1993. Muscle histochemical and biochemical properties in relation to meat quality during selection for increased lean tissue growth rate in pigs. J. Anim. Sci. 71:930-938.
Kauffman R.G., van Laack R.L.J.M., Russell R.L., Pospiech E., Cornelius C.A., Suckow C.E. and Greaser M.L. 1998. Can pale, soft, exudative pork be prevented by postmortem sodium bicarbonate injection? J. Anim. Sci. 76(12):3010-3015.
Kauffman R.G., Eikelenboom G., Van Derwal P.G., Merkus G. and Zaar M. 1986. The use of filter paper to estimate drip loss of porcine musculature. Meat Sci. 18:191-200.
Kazemi S., Ngadi M.O. and Gariépy C. 2011. Protein denaturation in pork longissimus muscle of different quality groups. Food Bioproc. Tech. 4:102-106.
Lefaucheur L. 2006. Myofibre typing and its relationships to growth performance and meat quality. Arch. Tierz., Dummerstorf 49 Special Issue. p. 04-17.
Leseigneur-Meynier A. and Gandemer G. 1991. Lipid composition of pork muscle in relation to the metabolic type of the fibres. Meat Sci. 29:229-241.
Macleod G. 1998. The flavour of beef. In: F. Shahidi, editor, Flavor of meat, meat products and seafoods. 2nd. ed. Blackie academic and professional, London, UK. p. 27-60.
Maltin C.A., Sinclair K.D., Warriss P.D., Grant C.M., Porter A.D. and Delday M.I. 1998. The effects of age at slaughter, genotype and finishing system on the biochemical properties, muscle fibre type characteristics and eating quality of bull beef from suckled calves. Anim Sci. 66:341-348.
Meilgaard M., Civille G.V. and Carr, B.T. 1991. Affective tests: Consumer tests and inhouse panel acceptance tests. Sensory evaluation techniques. pp. 211-222. (3rd edition). Boca Ranton. FL: CRC Press Inc.
Meynier A. and Gandemer G. 1994. La flaveur des viandes cuites: relations avec l'oxydation des phospholipides. Viandes Produits Carnés 15:179-182.
Moody W.G. 1983. Beef flavor -A review. Food Technol. 37:227-232.
Mottram D.S. and Edwards R. A. 1983. The role of triglycerides and phospholipids in the aroma of cooked beef. J. Sci. Food Agric. 34:517-522.
Muchenje V., Dzama K., Chimonyo M., Strydom P.E., Hugo A. and Raats J.G. 2008. Sensory evaluation and its relationship to physical meat quality attributes of beef from nguni and bonsmara steers raised on natural pasture. Anim. 2:1700-1706.
Nam Y.J., Choi Y.M., Lee S.H., Choe J.H., Jeong D.W., Kim Y.Y. and Kim B.C. 2009. Sensory evaluations of porcine longissimus dorsi muscle: relationships with postmortem meat quality traits and muscle fiber characteristics. Meat Sci. 83:731-736.
Nppc. 2000. Pork composition and quality assessment procedures. Des Moines, Ia. National pork producers council.
NRC. 1998. Nutrient requirements of swine (10th edition). Washington, DC, USA: National Academy Press.
Ockerman H.W., Jaworek D., Vanstavern B., Parrett N. and Pearson C.J. 1984. Castration and sire effects on carcass traits, meat palatability and muscle fibre characteristics in angus cattle. J. Anim. Sci. 59: 981-990.
Offer G. 1991. Modeling of the formation of pale, soft and exudative meat: Effects of chilling regime and rate and extent of glycolysis. Meat Science, 30(2):157-184.
Offer G. and Knight P. 1988. The structural basis of water-holding in meat. In: R. Lawrie, editor, Developments in meat science. Elsevier Applied Science, London, UK. pp. 63-243.
Orzechowska B., Wojtysiak D., Migda? W. and Tyra M. 2008. Relationships between muscle fibre characteristics and physico-chemical properties of longissimus lumborum muscle and growth rate in pig fasteners of three breeds. Anim. Sci. Pap. Rep. 26:277-285.
Oury M.P., Picard B., Briand M., Blanquet J.P. and Dumont R. 2009. Interrelationships between meat quality traits, texture measurements and physicochemical characteristics of M. Rectus abdominis from charolais heifers. Meat Sci. 83:293-301.
Peter J.B., Barnard R.J., Edgerton V.R., Gillespie C.A. and Stemple K.E. 1972. Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry. 11:2627-2633.
Reg. EC 1/2005; directives 74/577/EEC; Law 439 2 August 1978.
Renand G., Picard B., Touraille C., Berge P. and Lepetit J. 2001. Relationships between muscle characteristics and meat quality traits of young charolais bulls. Meat Sci. 59:49-60.
Ryu Y.C. and Kim B.C. 2006. Comparison of histochemical characteristics in various pork groups categorized by postmortem metabolic rate and pork quality. J. Anim. Sci. 84:894-901.
Ruiz-Carrascal J., Ventanas J., Cava R., Andrés A. I. and García C. 2000. Texture and appearance of dry cured ham as affected by fat content and fatty acid composition. Food Res. Intern. 33:91-95.
Sazili A.Q., Parr T., Sensky P.L., Jones S.W., Bardsley R.G. and Buttery, P.J. 2005. The relationship between slow and fast myosin heavy chain content, calpastatin and meat tenderness in different ovine skeletal muscles. Meat. Sci. 69:17-25.
Schiaffino S. and Reggiani C. 1996. Molecular diversity of myofibrillar proteins: gene regulation and functional significance. Physiol. Rev. 76:371-423.
Strydom P.E., Naude R.T., Smith M.F., Scholtz M.M. and Van Wyk J.B. 2000. Characterisation of indigenous african cattle breeds in relation to meat quality traits. Meat Sci. 55:79-88.
Talmadge R.J. and Roy R.R. 1993. Electrophoretic separation of rat skeletal muscle myosin heavy chain isoforms. J. Applied Physiol. 75:2337-2340.
Taylor R.G. 2004. Muscle fiber types and meat quality. Encyclopedia of Meat Science. p. 2:876-882.
Totland G.K., Kryvi H. and Slinde E. 1988. Composition of muscle fibre types and connective tissue in bovine m. Semitendinosus and its relation to tenderness. Meat Sci. 23: 303.
Velotto S., Vitale C. and Crasto A. 2012. Muscle fibre types, fat deposition and fatty acid profile of casertana versus large white pig. Anim. Sci. Pap. Rep. 30:35-44.
Wu W., Gao X. G., Dai Y., Fu Y., Li X.M. and Dai R.T. 2015. Post-mortem changes in sarcoplasmic proteome and its relationship to meat color traits in M. semitendinosus of Chinese Luxi yellow cattle. Food. Res. Int. 72:98-105.
Zelechowska E., Przybylsk W. and Jaworska D. 2012. Technological and sensory pork quality in relation to muscle and drip loss protein profiles. Eur. Food Res. Tech. 234:883-89.
Zheng Y., Wang S. and Yan P. 2018. The meat quality, muscle fiber characteristics and fatty acid profile in Jinjiang and F1 Simmental× Jinjiang yellow cattle. Asian-Australas. J. Anim Sci. 31(2):301.