Evaluation of the durum wheat landrace genetic diversity using agro analysis and its benefit for human health

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Mohammad Ihsan
Arshad Khan
Nausheen Nazir
Mohammad Nisar
Tour Jan
Shariat Ullah
Tariq Aziz
Fahad Al-Asmari
Sahar A. Alshareef
Maha Aljabri
Manal Y. Sameeh


Genetic diversity, Durum wheat, morphology, genotypes, SDS-PAGE


Ancient wheat and landraces (traditional varieties) are fascinating to the consideration of scientists who are re-evaluating the healthy and dietary properties attributed to them by popular tradition. Wheat (Triticum durum) is one of the most significant crops that is grown all over the world. In the present study, 19 landraces were analyzed for genetic diversity through morphological and SDS-PAGE analysis. The highest coefficient of variance (18.91%) was found for spike length, and the lowest for days to maturity (1.51%). The genotype Malakand and Dolram produced a maximum number of seeds (42 and 46 seeds per plant), and the genotypes Dogai and Rasool Banda were found to be the tallest (73 and 71 cm, respectively). The genotype Kamar Kotki was found to mature early (165 days) while Doagi was found to mature late (171 days). Spike length was found to be positively correlated with plant height, leaf width, and leaf length. Leaf width was significant with spike length and the internode distance. The cluster analysis divided all the genotypes into six sub-clusters where the Asban and the Shekhan were placed at the extreme of the dendrogram. Based on the total seed storage proteins analysis, 18 (85.71%) bands were polymorphic while 14.28% bands were found to be monomorphic. Using Ward’s methods, all the genotypes were divided into two main lineages at 25% Euclidian distance and five sub-clusters at 75% distance. Our findings have significance for understanding the variations and relationships between various durum wheat genotypes and could serve as a basis for local elite durum germplasm that has been regionally adapted.

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Aghaee M., Mohammadi R. and Nabovati S. 2010. Agro-morphological characterization of durum wheat accessions using pattern analysis. Australian Journal of Crop Science. 4(7):505–514. 10.3316/informit.536537388993889

Ahmad M., Dar Z.A. and Habib M. 2014. A review on oat (Avena sativa L.) as a dual-purpose crop. Scientific Research and Essays. 9(4):52–59. 10.5897/SRE2014.5820

Awulachew M.T. 2020. Understanding basics of wheat grain and flour quality. Journal of Health and Environmental Research. 6(1):10–26. 10.11648/j.jher.20200601.12

Barcaccia G., 2009. Molecular markers for characterizing and conserving crop plant germplasm. Molecular Techniques in Crop Improvement: 2nd Edition, pp. 231–254. 10.1007/978-90-481-2967-6_10

Bauer I., Drinic S.M., Drinić G. and Micić D.I. 2007. Assessing temporal changes in genetic diversity of maize hybrids using RAPD markers. Cereal Research Communications. 35(4):1563–1571. 10.1556/CRC.35.2007.4.3

El-Rawy M.A. 2020. Assessment of genetic diversity for some Egyptian wheat varieties based on morphological characters and SSR markers. Scientific Journal of Agricultural Sciences. 2(2):144–160. 10.21608/SJAS.2020.37530.1033

Feuillet C., Langridge P. and Waugh R. 2008. Cereal breeding takes a walk on the wild side. Trends in genetics. 24(1): 24–32. 10.1016/j.tig.2007.11.001

Fufa H.U.N.D.E.R.A., Baenziger P.S., Beecher B.S., Dweikat I., Graybosch R.A. and Eskridge K.M. 2005. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica. 145: 133–146. 10.1007/s10681-005-0626-3

Ghafoor A., Ahmad Z., Qureshi A.S. and Bashir M. 2002. Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica. 123: 367–378. 10.1023/A:1015092502466

Ghasemi N., Mirfakhraii R.G. and Abbasi A. 2019. Assessment of genetic diversity of bread wheat (Triticum aestivum L.) cultivars using microsatellite markers. Journal of Crop Breeding. 11(29): 9–16. 10.29252/jcb.11.29.9

Hassan M.I. 2016. Assessment of genetic diversity in bread wheat genotypes based on heat tolerance and SSR markers. Assiut Journal of Agricultural Sciences. 47(5). 10.21608/AJAS.2016.1987

Ihsan M., Nazir N., Ghafoor A., Khalil A.A.K., Zahoor M., Nisar M., et al. 2021. Genetic diversity in local and exotic Avena sativa L. (Oat) germplasm using multivariate analysis. Agronomy. 11(9): 1713. 10.3390/agronomy11091713

Kesralikar M., Mokate S., Golakiya B.A. and Vakharia D.N. 2017. Genetic diversity analysis among onion genotypes through RAPD, SSR and ISSR markers. International Journal of Current Microbiology and Applied Sciences. 6(6): 1467–1474. 10.20546/ijcmas.2017.606.172

Khaled A.G.A., Motawea M.H. and Said A.A. 2015. Identification of ISSR and RAPD markers linked to yield traits in bread wheat under normal and drought conditions. Journal of Genetic Engineering and Biotechnology. 13(2): 243–252. 10.1016/j.jgeb.2015.05.001

Khan A., Ihsan M., Nisar M., Hazrat A., Ali M., Ul-Haq R., et al. 2021. Evaluation of Genetic Diversity in Barley Landraces through Agro-Morphological and Biochemical Characterization. Sarhad Journal of Agriculture. 37. 10.17582/journal.sja/2021/37.3.984.992

Khan J., Khurshid S., Sarwar A., Aziz T., Naveed M., Ali U., et al. 2022. Enhancing Bread Quality and Shelf Life via Glucose Oxidase Immobilized on Zinc Oxide Nanoparticles—A Sustainable Approach towards Food Safety. Sustainability. 14(21): 14255. 10.3390/su142114255

Khan M.K., Pandey A., Thomas G., Akkaya M.S., Kayis S.A., Ozsensoy Y., et al. 2015. Genetic diversity and population structure of wheat in India and Turkey. AoB Plants. 7: 83. 10.1093/aobpla/plv083

Khodadadi M., Fotokian M.H. and Miransari M., 2011. Genetic diversity of wheat (Triticum aestivum L.) genotypes based on cluster and principal component analyses for breeding strategies. Australian Journal of Crop Science. 5(1): 17–24.

Kraic F.I.L.I.P., Mocák J., Roháčik T.I.B.O.R. and Sokolovičová J.A.N.A. 2009. Chemometric characterization and classification of new wheat genotypes. Nova Biotechnol. 9:101–106.

Malik R., Sharma H., Sharma I., Kundu S., Verma A., Sheoran S., Kumar R. and Chatrath R. 2014. Genetic diversity of agro--morphological characters in Indian wheat varieties using GT biplot. Australian Journal of Crop Science. 8(9): 1266–1271.

Niaz S., Ahmad H., Khan I.A., Shah A.H. and Rahman I. 2014. Molecular and agronomic characterization of durum wheat. Intl. J. Biosci. 4: 270–275. 10.12692/ijb/4.7.270-275

Nisar M., Ghafoor A., Khan M.R. and Subhan M. 2009. Genetic similarity of Pakistani pea (Pisum sativum L.) germplasm with world collection using cluster analysis and jaccard’s similarity index. Journal of the Chemical Society of Pakistan. 31(1): 138–144.

Okii D., Tukamuhabwa P., Odong T., Namayanja A., Mukabaranga J., Paparu P. and Gepts P., 2014. Morphological diversity of tropical common bean germplasm. African Crop Science Journal. 22(1): 59–68.

Saleem B., Khan A.S., Shahzad M.T. and Ijaz F. 2016. Estimation of heritability and genetic advance for various metric traits in seven F2 populations of bread wheat (Triticum aestivum L.). Journal of Agricultural Sciences. 61(1): 1–9. 10.2298/JAS1601001S

Salem K.F., Röder M.S. and Börner A. 2015. Assessing genetic diversity of Egyptian hexaploid wheat (Triticum aestivum L.) using microsatellite markers. Genetic resources and crop evolution. 62: 377–385. 10.1007/s10722-014-0159-5

Shuaib M., Zeb A., Ali Z., Ali W., Ahmad T. and Khan I. 2007. Characterization of wheat varieties by seed storage protein electrophoresis. African Journal of Biotechnology. 6(5).

Siahbidi M.M.P., Aboughadareh A.P., Tahmasebi G.R., Teymoori M. and Jasemi M. 2013. Evaluation of genetic diversity and interrelationships of agro-morphological characters in durum wheat (Triticum durum Desf.) lines using multivariate analysis. International Journal of Agriculture. 3(1): 184.

Varzakas T., Kozub N. and Xynias I.N. 2014. Quality determination of wheat: genetic determination, biochemical markers, seed storage proteins–bread and durum wheat germplasm. Journal of the Science of Food and Agriculture. 94(14): 2819–2829. 10.1002/jsfa.6601

Zahoor M., Nisar M. and Islam N. 2015. Genetic variations of Robinia pseudoacacia plant using SDS-PAGE. Pak. J. Bot. 46(6): 2335–2338.

Zarkti H., Ouabbou H., Hilali A. and Udupa S.M. 2010. Detection of genetic diversity in Moroccan durum wheat accessions using agro-morphological traits and microsatellite markers. African Journal of Agricultural Research. 5(14): 1837–1844. 10.5897/AJAR09.249

Zhang W. and Cai X. 2019. Alien introgression and breeding of synthetic wheat. In Advances in breeding techniques for cereal crops (pp. 3–54). Burleigh dodds science publishing.

Zubair M., Ajmal S.U., Anwar M. and Haqqani A.M. 2007. Multivariate analysis for quantitative traits in mungbean [Vigna radiata (L.) Wilczek]. Pakistan Journal of Botany. 39(1): 103.