COMPARATIVE 1D- AND 2D-ELECTROPHORETIC PROTEIN PROFILES OF ANCESTRAL AND MODERN BUCKWHEAT SEEDS GROWN IN THE ITALIAN ALPINE REGION

Main Article Content

J. CAPRARO
C. MAGNI
A. GIORGI
M. DURANTI
A. SCARAFONI

Keywords

buckwheat, electrophoresis, Fagopyrum spp., historical landraces, proteome, traditional cultivation

Abstract

Buckwheat is an old crop whose seeds protein composition candidates these under-utilised seeds as much needed ingredients for the production of gluten-free products. Several buckwheat species and local cultivars are known worldwide. In this work we used 1D and 2D electrophoresis to characterize and compare the seed protein profiles of two buckwheat species (Fagopyrum esculentum and Fagopyrum tataricum). The two analyzed cultivars of F. esculentum represent authentic landraces of an Italian Alpine valley, named Valtellina. The protein profiles of F. tataricum and the two F. esculentum cultivars didn’t show major differences. However, narrow but significant differences were present also between these two landraces, allowing their discrimination at protein level. This work represents a molecular based approach for the designation of origin and authenticity of local buckwheat varieties and tracing them in flours for human food.

Abstract 301 | pdf Downloads 219

References

Barcaccia G., Volpato M., Gentili R., Abeli T., Galla G., Orsenigo S., Citterio S., Sgorbati S. and Rossi G. 2016. Genetic identity of common buckwheat (Fagopyrum esculentum Moench) landraces locally cultivated in the Alps. Genet. Resour. Crop Evol. 63: 639.

Capraro J., Magni C., Fontanesi M., Budelli A. and Duranti M. 2008. Application of two-dimensional electrophoresis to industrial process analysis of proteins in lupin-based pasta. LWT -Food Sci. Technol. 41: 1011.

Casey R., Domoney C. and Ellis N. 1985. Legume storage proteins and their genes. Oxford Surv. Plant Mol. Cell Biol. 3: 1.

Du S.K., Li Z.X. and Yu X.Z. 2004. Research progress on buckwheat protein. Food Sci. 10: 409.

Duranti M. and Scarafoni A. 2015. Can legume seed proteins be a valid alternative to animal sources to fulfill the growing need of food proteins? Agro Food Ind. Hi. Tech. 26: 8.

Eggum B.O., Kreft I., Javornik B. 1980. Chemical composition and protein quality of buckwheat (Fagopyrum esculentum Moench). Plant Foods Hum. Nutr. 30: 175.

Ferranti R., Pirola A. and Penati F. 2002. Il paesaggio vegetale della Provincia di Sondrio, Suppl. Il Naturalista Valtellinese, Atti del Museo Civico di Storia Naturale di Morbegno 13: 38.

Gorinstein S., Drzewiecki J., Delgado Licon E., Pawelzik E., Martinez Ayala A.L., Medina O.J., Haruenkit R. and Trakhtenberg S. 2005. Relationship between dicotyledone-amaranth, quinoa, fagopyrum, soybean and monocots-sorghum and rice-based on protein analyses and their use as substitution of each other. Eur. Food Res. Technol. 221(1): 69.

Izydorczyk M.S., McMillan T., Bazin S., Kletke J., Dushnicky L. and Dexter J. 2014. Canadian buckwheat: A unique, useful and under-utilized crop. Can.J. Plant Sci. 94: 509.

Kreft S., Strukelj B., Gaberscik A. and Kreft I. 2002. Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method. J. Exp. Bot. 53: 1801.

Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 660.

Li J.H., Chen Q.F. and Zeller F.J. 2008. Variation in seed protein subunits among species of the genus Fagopyrum Mill. Plant Syst. Evol. 274: 193.

Li S.Q. and Zhang Q.H. 2001. Advances in the Development of Functional Foods from Buckwheat. Crit. Rev. Food Sci. Nutr. 41: 451.

Magni C., Scarafoni A., Herndl A., Sessa F., Prinsi B., Espen L. and Duranti M. 2007. Combined 2D electrophoretic approaches for the study of white lupin mature seed storage proteome. Phytochem. 68(7): 997.

Nailecz D., Dziuba J., Minkiewicz P., Dziuba M. and Szerszunowicz I. 2009. Identification of oat (Avena sativa) and buckwheat (Fagopyrum esculentum) proteins and their prolamin fractions using two-dimensional polyacrylamide gel electrophoresis. Eur. Food Res. Technol. 230: 71.

Ohnishi O. and Matsuoka Y. 1996. Search for the wild progenitor of buckwheat II. Taxonomy of Fagopyrum (Polygonaceae) species based on morphology, isozymes and cpDNA variability. Genes Syst. 71: 383.

Ohnishi O. 1993. Population genetics of cultivated common buckwheat, Fagopyrum esculentum Moench. VIII. Local differentiation of land races in Europe and the silk road. Jap. J. Genet. 68: 303.

Radovic S.R., Maksimovic V.R. and Varkonji-Gašic E.I. 1996. Characterization of buckwheat seed storage proteins. J. Agric. Food Chem. 44(4): 972.

Rout A. and Chrungoo N.K. 2007. Genetic variation and species relationships in Himalayan buckwheats as revealed by SDS PAGE of endosperm proteins extracted from single seeds and RAPD based DNA fingerprints. Genet. Res. Crop. Evol. 54: 767.

Scarafoni A., Magni C. and Duranti M. 2007. Molecular nutraceutics as a mean to investigate the positive effects of legume seed proteins on human health. Trends Food Sci. Tech. 18(9): 454.

Yasui Y. and Ohnishi O. 1998. Phylogenetic relationships among Fagopyrum species revealed by the nucleotide sequences of the ITS region of the nuclear rRNA gene. Genes Genet. Syst. 73: 201.

Zeller F.J., Weishaeupl H. and Hsam S.L.K. 2004. Identification and genetics of buckwheat (Fagopyrum) seed storage protein. In: Seung S.H., Choi Y.S., Kim N.S., Proceedings of the VIII International Symposium on buckwheat, Chunchon, Korea. Advances in buckwheat research: 195.

Zhou X., Wen L., Li Z., Zhou Y., Chen Y. and Lu Y. 2015. Advances on the benefits of bioactive peptides from buckwheat. Phytochem. Rev. 14: 381.